SEARCH

PAGETOP

協働研究所

日本電子YOKOGUSHI協働研究所

難波 啓一 特任教授 難波 啓一 特任教授

キーワード:

クライオ電子顕微鏡、超分子立体構造、べん毛モーター、蛋白質輸送、筋収縮、ナノマシン

クライオ電子顕微鏡法による構造生命科学の発展

細菌べん毛、細胞骨格、筋繊維など、生体運動やタンパク質輸送を駆動する分子モーター関連超分子や膜タンパク質を含む分子ナノマシンについて、その自己集合、力発生、動作制御、エネルギー変換など、高精度でダイナミックな動作のしくみを原子・分子レベルで解明する。クライオ電子顕微鏡法によるデータ収集と画像解析をより高速化することにより、生体分子の立体構造解析法を高度化し、様々な超分子ナノマシンの原子構造から動作原理を解明して、創薬やナノマシン設計など、将来のバイオナノテクノロジーの基盤技術確立に役立てる。

(左)細菌の運動器官べん毛はらせん型プロペラで、基部のモーターにより毎分2万回も回転して推進力を発生。べん毛はその構成タンパク質が基部の輸送装置で先端へ送られ先端成長する。(右)クライオ電子顕微鏡法は生体超分子を構成分子にばらして結晶化する必要がなく、超分子の機能を持ったままの立体構造とその変化を観察できる技術として生命科学の強力な武器になりつつある。

メンバー

難波 啓一 特任教授 keiichi[at]fbs.osaka-u.ac.jp
宮田 知子 特任准教授(常勤) miya[at]fbs.osaka-u.ac.jp
木下 実紀 特任助教(常勤) miki[at]fbs.osaka-u.ac.jp
藤田 純三 特任助教(常勤) jfujita[at]fbs.osaka-u.ac.jp
荒川 真 特任研究員(常勤)  
牧野 文信 招へい准教授  
守矢 香奈 招へい研究員  
阿部 康代 技術補佐員  
山田 智絵 技術補佐員  
串間 由江 技術補佐員  
山口 智子 大学院生  
   

研究者の詳細を大阪大学研究者総覧Research Mapで検索できます。

  • ※メールアドレスの[at]は@に変換してください

研究成果

論文、総説、著書

2021年

Matsunami, H., Yoon, Y.-H., Imada, K., Namba, K. & Samatey, F.A.

Structure of the bacterial flagellar hook cap provides insights into a hook assembly mechanism.

Communications Biology 4; 1291  2021 DOI:10.1038/s42003-021-02796-6

Minamino, T., Morimoto, Y.V., Kinoshita, M. & Namba, K.

Multiple roles of flagellar export chaperones for efficient and robust flagellar filament formation in Salmonella.

Frontiers in Microbiology 12; 756044  2021 PMID:34691007 DOI:10.3389/fmicb.2021.756044

Hendriksen, J.J., Lee, H.J., Bradshaw, A.J., Namba, K., Chevance, F.F.V., Minamino, T., Hughes, K.T.

Genetic analysis of the Salmonella FliE protein that forms the base of the flagellar axial structure.

mBio 12(5): e02392-21  2021 PMID:34579566 DOI:10.1128/mBio.02392-21

Jin, F., Sun, M., Fujii, T., Yamada, Y., Wang, J., Maturana, A.D., Wada, M., Su, S., Ma, J., Takeda, H., Kusakizako, T., Tomita, A., Nakada-Nakura, Y., Liu, K., Uemura, T., Nomura, Y., Nomura, N., Ito, K., Nureki, O., Namba, K., Iwata, S., Yu, Y. & Hattori, M.

The structure of MgtE in the absence of magnesium provides new insights into channel gating.

PLoS Biol. 19(4): e3001231  2021 PMID:33905418 DOI:10.1371/journal.pbio.3001231

Toyonaga, T., Kato, T., Kawamoto, A., Kodera, N., Hamaguchi, T., Tahara, Y.O., Ando, T., Namba, K. & Miyata, T.

Chained structure of dimeric F1-like ATPase in Mycoplasma mobile gliding machinery.

mBio 12(4): e0141421  2021 PMID:34281395 DOI:10.1128/MBIO.01414-21

Yamaguchi, T., Makino, F., Miyata, T., Minamino, T., Kato, T. & Namba, K.

Structure of the molecular bushing of the bacterial flagellar motor.

Nat. Commun. 12, 4469  2021 PMID:34294704 DOI:10.1038/s41467-021-24715-3

Kawamoto, A., Miyata, T., Makino, F., Kinoshita, M., Minamino, T., Imada, K., Kato, T. & Namba, K.

Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries.

Nat. Commun. 12, 4223  2021 PMID:34244518 DOI:10.1038/s41467-021-24507-9

Minamino, T., Morimoto, Y.V., Kinoshita, M. & Namba, K.

Membrane voltage-dependent activation mechanism of the bacterial flagellar protein export apparatus.

PNAS 118 (22) e2026587118  2021 PMID:34035173 DOI:10.1073/pnas.2026587118

Minamino, T. & Namba, K.

Recent advances in the bacterial flagellar motor study

Biomolecules 11, 741  2021 PMID:34067523 DOI:10.3390/biom11050741

Inoue, Y., Kinoshita, M., Kida, M., Takekawa, N., Namba, K., Imada, K. & Minamino, T.

The FlhA linker mediates flagellar protein export switching during flagellar assembly.

Communications Biology 4, 646  2021 PMID:34059784 DOI:10.1038/s42003-021-02177-z

Kinoshita, M., Namba, K. & Minamino, T.

A positive charge region of Salmonella FliI is required for ATPase formation and efficient flagellar protein export.

Communications Biology 4, 464  2021 PMID:33846530 DOI:10.1038/s42003-021-01980-y

Minamino, T., Kinoshita, M., Morimoto, Y.V. & Namba, K.

The FlgN chaperone activates the Na+-driven engine of the Salmonella flagellar protein export apparatus.

Communications Biology 4, 335  2021 PMID:33712678 DOI:10.1038/s42003-021-01865-0

Takekawa, N., Kawamoto, A., Sakuma, M., Kato, T., Kojima, S., Kinoshita, M., Minamino, T., Namba, K., Homma, M. & Imada, K.

Two distinct conformations in 34 FliF subunits generate three different symmetries within the flagellar MS-ring.

mBio 12:e03199-20  2021 PMID:33653894 DOI:10.1128/mBio.03199-20.

Coruh, O., Frank, A., Tanaka, H., Kawamoto, A., El-Mohsnawy, E., Kato, T., Namba, K., Gerle, C., Nowaczyk, M.M., Kurisu, G.

Cryo-EM structure of a functional monomeric Photosystem I from Thermosynechococcus elongates reveals ‘red’ chlorophyll cluster.

Commun. Biol. 4, 304  2021 PMID:33686186 DOI:10.1038/s42003-021-01808-9

Inoue, Y., Hanazono, Y., Noi, K., Kawamoto, A., Kimatsuka, M. Harada, R.,Takeda, K., Iwamasa, N., Shibata, K., Noguchi, K., Shigeta, Y., Namba, K., Ogura, T., Miki, K., Shinohara, K., Yohda, M.

Split conformation of Chaetomium thermophilum Hsp104 disaggregase.

Structure 29, 1-10  2021 PMID:33651974 DOI:10.1016/j.str.2021.02.002

Ariyoshi, M., Makino, F., Watanabe, R., Nakagawa, R., Kato, T., Namba, K., Arimura, Y., Fujita, R., Kurumizaka, H., Okumura, E., Hara, M. & Fukagawa, T.

Cryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C.

EMBO Journal e105671  2021 PMID:33463726 DOI:10.15252/embj.2020105671

Morimoto, Y.V. & Minamino, T.

Architecture and assembly of the bacterial flagellar motor complex.

Macromolecular Protein Complexes III: Structure and Function, Subcellular Biochemistry 96: 297-321  2021 PMID:33252734 DOI:10.1007/978-3-030-58971-4_8.

2020年

Fulano, A.M., Shen, D., Zhang, E.-H., Shen, X., Chou, S.-H., Minamino, T., Puopolo, G. & Qian, G.

Functional divergence of flagellar type III secretion system: A case study in a non-flagellated, predatory bacterium.

Comput. Struct. Biotechnol. 18, 3368-3376  2020 DOI:10.1016/j.csbj.2020.10.029

Terahara, N., Namba, K. & Minamino, T.

Dynamic exchange of two types of stator units in Bacillus subtilis flagellar motor in response to environmental changes.

Comput. Struct. Biotechnol. 18, 2897-2907  2020 PMID:33163150 DOI:10.1016/j.csbj.2020.10.009

Vizarraga, D., Kawamoto, A., Matsumoto, U., Illanes, R., Perez-Luque, R., Martin, J., Mazzolini, R., Bierge, P., Pich, O., Espasa, M., Sanfeliu, I., Esperalba, J., Fernandez-Huerta, M., Scheffer, M., Pinyol, J., Frangakis, A., Lluch-Senar, M., Mori, S., Shibayama, K., Kenri, T., Kato, T., Namba, K., Fita, I., Miyata, M. & Aparicio, D.

Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae.

Nat. Commun. 11, 5188  2020 PMID:33057023 DOI:10.1038/s41467-020-18777-y

Morimoto, V.Y., Namba, K. & Minamino, T.

GFP fusion to the N-terminus of MotB affects the proton channel activity of the bacterial flagellar motor in Salmonella.

Biomolecules 10, 1255  2020 PMID:32872412 DOI:10.3390/biom10091255

Kishikawa, J., Nakanishi, A., Furuta, A., Kato, T., Namba, K., Tamakoshi, M., Mitsuoka, K. & Yokoyama, K.

Mechanical inhibition of isolated V0 from V/A-ATPase for proton conductance.

eLife 9:e56862  2020 PMID:32639230 DOI:10.7554/eLife.56862

Terashima, H., Hirano, K., Inoue, Y., Tokano, T., Kawamoto, A., Kato, T., Yamaguchi, E., Namba, K., Uchihashi, T., Kojima, S. & Homma, M.

Assembly mechanism of a supramolecular MS-ring complex to initiate bacterial flagellar biogenesis in Vibrio species.

J. Bacteriol. 202, e00236-20  2020 PMID:32482724 DOI:10.1128/JB.00236-20

Fulano, A.M., Shen, D., Kinoshita, M., Chou, S.-H., & Qian, G.

The homologous components of flagellar type III protein apparatus have acquired a novel function to control twitching motility in a non-flagellated biocontrol bacterium.

Biomolecules 10, 733  2020 PMID:32392834 DOI:10.3390/biom10050733

Miyata, M., Robinson, R.C., Uyeda, T.P.Q., Fukumori, Y., Fukushima, S., Haruta, S., Homma, M., Inaba, K., Ito, M., Kaito, C., Kato, K., Kenri, T., Kinoshita, Y., Kojima, S., Minamino, T., Mori, H., Nakamura, S., Nakane, D., Nakayama, K., Nishiyama, M., Shibata, S., Shimabukuro, K., Tamakoshi, M., Taoka, A., Tashiro, Y., Tulum, I., Wada, H. & Wakabayashi, K.

Tree of motility _ A proposed history of motility systems in the tree of life.

Genes Cells 25:6_21  2020 PMID:31957229 DOI:10.1111/gtc.12737

Oide, M., Kato, T., Oroguchi, T. & Nakasako, M.

Energy landscape of domain motion in glutamate dehydrogenase deduced from cryo-electron microscopy.

FEBS J. 287, 3472-3493  2020 PMID:31976609 DOI:10.1111/febs.15224

Nakamura, S., Hanaizumi, Y., Morimoto, Y.V., Inoue, Y., Erhardt, M., Minamino, T. & Namba, K.

Direct observation of speed fluctuations of flagellar motor rotation at extremely low load close to zero.

Mol. Microbiol. 113(4):755-765  2020 PMID:31828860 DOI:10.1111/mmi.14440

Yamaguchi, T., Toma, S., Terahara, N., Miyata, T., Ashihara, M., Minamino, T., Namba, K. & Kato, T.

Structural and functional comparison of Salmonella flagellar filaments composed of FljB and FliC.

Biomolecules 10, 246  2020 PMID:32041169 DOI:10.3390/bioml0020246

Minamino, T., Inoue, Y., Kinoshita, M. & Namba, K.

FliK-driven conformational rearrangements of FlhA and FlhB are required for export switching of the flagellar protein export apparatus.

J. Bacteriol. 202:e00637-19  2020 PMID:31712281 DOI:10.1128/JB.00637-19

Terashima, H., Tatsumi, C., Kawamoto, A., Namba, K., Minamino, T. & Imada, K.

In vitro autonomous construction of the flagellar axial structure in the inverted membrane vesicles.

Biomolecules 10, 126  2020 PMID:31940802 DOI:10.3390/biom10010126

Yamada, Y., Namba, K. & Fujii, T.

Cardiac muscle thin filament structures reveal calcium regulatory mechanism.

Nat. Commun. 11, 153  2020 PMID:31919429 DOI:10.1038/s41467-019-14008-1

2019年

Nishikawa, M.S., Nakane, D., Toyonaga, T., Kawamoto, A., Kato, T., Namba, K. & Miyata, M.

Refined mechanism of Mycoplasma mobile gliding based on structure, ATPase activity, and sialic acid binding of machinery.

mBio 10:e02846-19  2019 PMID:31874918 DOI:10.1128/mBio.02846-19

Kato, T., Makino, F., Miyata, T., Horv_th, P. & Namba, K.

Structure of the native supercoiled flagellar hook as a universal joint.

Nat. Commun. 10, 5295  2019 PMID:31757961 DOI:10.1038/s41467-019-13252-9

Kato, T., Makino, F., Nakane, T., Terahara, N., Kaneko, T., Shimizu, Y., Motoki, S., Ishikawa, I., Yonekura, K. & Namba, K.

CryoTEM with a cold emission gun that moves structural biology into a new stage.

Microsc. microanal. 25(Suppl 2):998-999  2019 DOI:10.1017/S1431927619005725

Horvath, P., Kato, T., Miyata, T. & Namba, K.

Structure of Salmonella flagellar hook reveals intermolecular domain interactions for the universal joint function.

Biomolecules 9, 462  2019 PMID:31505847 DOI:10.3390/biom9090462

Minamino, T., Kinoshita, M. & Namba, K.

Directional switching mechanism of the bacterial flagellar motor.

Comp. Struct. Biotechnol. J. 17:1075-1081  2019 PMID:31452860 DOI:10.1016/j.csbj.2019.07.020

Nakamura, S. & Minamino, T.

Flagella-driven motility of bacteria.

Biomolecules 9, 279  2019 PMID:31337100 DOI:10.3390/biom9070279

Saijo-Hamano, Y., Matsunami, H., Namba, K. & Imada, K.

Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella.

Biomolecules 9, 260  2019 PMID:31284631 DOI:10.3390/biom9070260

Inoue, Y., Kinoshita, M., Namba, K. & Minamino, T.

Mutational analysis of the C-terminal cytoplasmic domain of FlhB, a transmembrane component of the flagellar type III protein export apparatus in Salmonella.

Genes Cells 24(6):408-421  2019 PMID:30963674 DOI:10.1111/gtc.12684

Inoue, Y., Ogawa, Y., Kinoshita, M., Shimada, M., Kodera, N., Ando, T., Namba, K, Kitao, A., Imada, K. & Minamino, T.

Structural insights into the substrate specificity switching mechanism of the type III protein export apparatus.

Structure 27(6):965-976.e6  2019 PMID:31031200 DOI:10.1016/j.str.2019.03.017

Sakai, T., Miyata, T., Terahara, N., Mori, K., Inoue, Y., Morimoto, Y.V., Kato, T., Namba, K. & Minamino, T.

Novel insights into conformational rearrangements of the bacterial flagellar switch complex.

mBio 10:e00079-19  2019 PMID:30940700 DOI:10.1128/mBio.00079-19

2018年

Fujii, T, Namba, K.

Complementary use of electron cryomicroscopy and X-ray crystallography: structural studies of actin and actomyosin filaments.

Integrative Structural Biology with Hybrid Methods, Advances in Experimental Medicine and Biology, Springer Nature Singapore Pte Ltd. 1105:25-42  2018 PMID:30617822 DOI:10.1007/978-981-13-2200-6_4

Minamino, T., Terahara, N., Kojima, S. & Namba, K.

Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment.

Mol. Microbiol. 10:723-734  2018 PMID:30069936 DOI:10.1111/mmi.14092

Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T. & Imada, K.

In vitro reconstitution of functional type III protein export and insights into flagellar assembly.

mBio 9(3):e00988-18  2018 PMID:29946050 DOI:10.1128/mBio.00988-18

Tahara, H., Takabe, K., Sasaki, Y., Kasuga, K., Kawamoto, A., Koizumi, N., Nakamura, S.

The mechanism of two-phase motility in the spirochete Leptospira: Swimming and crawling.

Sci. Adv. 4(5):eaar7975  2018 PMID:29854948 DOI:10.1126/sciadv.aar7975

Sasaki, Y., Kawamoto, A., Tahara, H., Kasuga, K., Sato, R., Ohnishi, M., Nakamura, S., Koizumi, N.

Leptospiral flagellar sheath protein FcpA interacts with FlaA2 and FlaB1 in Leptospira biflexa.

PLoS One 13(4):e0194923  2018 PMID:29634754 DOI:10.1371/journal.pone.0194923

Minamino, T.

Hierarchical protein export mechanism of the bacterial flagellar type III protein export apparatus.

FEMS Microbiol. Lett. 365:1-9  2018 PMID:29850796 DOI:10.1093/femsle/fny117

Namba, K., Kato, T.

Technical Development of Electron Cryomicroscopy and Contributions to Life Sciences.

JEOL NEWS 53:18-24  2018

Minamino, T., Kato, T., Makino, F., Horvath, P., Miyata, T. & Namba, K.

Electron Microscopy of Motor Structure and Possible Mechanisms.

Encyclopedia of Biophysics   2018 DOI:10.1007/978-3-642-16712-6_196

Minamino, T., Morimoto, Y.V., Kawamoto, A., Terashima, H. & Imada, K.

The bacterial flagellum.

InTechOpen 42811  2018 DOI:10.5772/intechopen.73277

Terahara, N., Inoue, Y., Kodera, N., Morimoto, Y.V., Uchihashi, T., Imada, K., Ando, T., Namba, K. & Minamino, T.

Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export.

Sci. Adv. 4(4):eaao7054  2018 PMID:29707633 DOI:10.1126/sciadv.aao7054

Kinoshita, M., Namba, K. & Minamino, T.

Effect of a clockwise-locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor.

Genes Cells 23:241-247  2018 PMID:29405551 DOI:10.1111/gtc.12565

Fujii, T., Matsunami, H., Inoue, Y. & Namba, K.

Evidence for the hook supercoiling mechanism of the bacterial flagellum.

Biophysics and Physicobiology 15:28-32  2018 PMID:29607277 DOI:10.2142/biophysico.15.0_28

Inoue, Y., Morimoto, Y.V., Namba, K. & Minamino, T.

Novel insights into the mechanism of well-ordered assembly of bacterial flagellar proteins in Salmonella.

Sci Rep 8(1787)  2018 PMID:29379125 DOI:10.1038/s41598-018-20209-3

Kinoshita, M., Furukawa, Y., Uchiyama, S., Imada, K., Namba, K. & Minamino, T.

Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor.

BBRC 496:12-17  2018 PMID:29294326 DOI:10.1016/j.bbrc.2017.12.118

Sakai, T., Inoue, Y., Terahara, N., Namba, K. & Minamino, T.

A triangular loop of domain D1 of FlgE is essential for hook assembly but not for the mechanical function.

BBRC 495:1789-1794  2018 PMID:29229393 DOI:10.1016/j.bbrc.2017.12.037

2017年

Terashima, N., Kawamoto, A., Morimoto, Y.V., Imada, K. & Minamino, T.

Structural differences in the bacterial flagellar motor among bacterial species.

Biophysics and Physicobiology 14:191-198  2017 PMID:29362704 DOI:10.2142/biophysico.14.0_191

Morimoto, Y.V., Kami-ike, N., Namba, K. & Minamino, T.

Determination of local pH differences within living Salmonella cells by high-resolution pH imaging based on pH-sensitive GFP derivative, pHluorin(M153R).

Bio-protocol 7(1):e2529  2017 DOI:10.21769/BioProtoc.2529

Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.

Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.

Sci. Adv. 3(11):eaao4119  2017 PMID:29109979 DOI:10.1126/sciadv.aao4119

Pourjaberi, S.N.S., Terahara, N., Namba, K. & Minamino, T.

The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.

Mol. Microbiol. 106(4):646-658  2017 PMID:28925530 DOI:10.1111/mmi.13843.

Takabe, K., Kawamoto, A., Tahara, H., Kudo, S. & Nakamura, S.

Implications of coordinated cell-body rotations for Leptospira motility.

Blood 491:1040-1046  2017 PMID:28780349 DOI:10.1016/j.bbrc.2017.08.007

Fukumura T, Makino F, Dietsche T, Kinoshita M, Kato T, Wagner S, Namba K, Imada K, Minamino T

Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex

PLoS. Biol. 15(8):e2002281  2017 PMID:28771466 DOI:10.1371/journal.pbio.2002281

Morimoto, Y.V., Minamino, T.

Stoichiometry and turnover of the stator and rotor.

The Bacterial Flagellum _ Methods and Protocols- 1593:203-213  2017 PMID:28389956 DOI:10.1007/978-1-4939-6927-2_16

Kawamoto, A., Namba, K.

Structural study of the bacterial flagellar basal body by electron cryomicroscopy and image analysis.

The Bacterial Flagellum _ Methods and Protocols- 1593:119-131  2017 PMID:28389949 DOI:10.1007/978-1-4939-6927-2_9

Kawamoto, A., Namba, K.

Fuel of the bacterial flagellar type III protein export apparatus.

The Bacterial Flagellum _ Methods and Protocols- 1593:3-16  2017 PMID:28389941 DOI:10.1007/978-1-4939-6927-2_1

Hiraoka, K.D., Morimoto, Y.V., Inoue, Y., Fujii, T., Miyata, T., Makino, F., Minamino, T. & Namba, K.

Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE.

Sci Rep 7:46723  2017 PMID:28429800 DOI:10.1038/srep46723.

Terahara, N., Noguchi, Y., Nakamura, S., Kamiike, N., Ito, M., Namba, K., Minamino, T.

Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor.

Sci Rep 7:46081  2017 PMID:28378843 DOI:10.1038/srep46081

Renault, T.T., Abraham, A.O., Bergmiller, T., Parados, G., Rainville, S., Charpentier, E., Guet, C.C., Tu, Y., Namba, K., Keener, J.P., Minamino, T. & Erhardt, M.

Bacterial flagella grow through an injection-diffusion mechanism.

eLife 6:e23136  2017 PMID:28262091 DOI:10.7554/eLife.23136

Morimoto, Y.V., Namba, K. & Minamino, T.

Measurements of free-swimming speed of motile Salmonella cells in liquid media.

Bio-protocol 7(1):e2093  2017 DOI:10.21769/BioProtoc.2093

Morimoto, Y.V., Namba, K. & Minamino, T.

Bacterial intracellular sodium ion measurement using CoroNa Green.

Bio-protocol 7(1):e2092  2017 DOI:10.21769/BioProtoc.2092

Fujii T, Kato T, Hiraoka KD, Miyata T, Minamino T, Chevance FF, Hughes KT, Namba K

Identical folds used for distinct mechanical functions of the bacterial flagellar rod and hook.

Nat. Commun. 8:14276  2017 PMID:28120828 DOI:10.1038/ncomms14276

Fujii T, Namba K.

Structure of actomyosin rigour complex at 5.2-_ resolution and insights into the ATPase cycle mechanism

Nat. Commun. 8:13969  2017 PMID:28067235 DOI:10.1038/ncomms13969

2016年

Morimoto YV, Kami-Ike N, Miyata T, Kawamoto A, Kato T, Namba K, Minamino T

High-resolution pH imaging of living bacterial cell to detect local pH differences.

mBio 7(6)  2016 PMID:27923921 DOI:10.1128/mBio.01911-16

Makino, F., Shen, D., Kajimura, N., Kawamoto, A., Pissaridou, P., Oswin, H., Pain M., Murillo, I., Namba, K. & Blocker, A.J.

The architecture of the cytoplasmic region of type III secretion systems.

Sci Rep 6:33341  2016 PMID:27686865 DOI:10.1038/srep33341

Furukawa, Y., Inoue, Y., Sakaguchi, A., Mori, Y., Fukumura, T., Miyata, T., Namba, K. & Minamino, T.

Structural stability of flagellin subunit affects the rate of flagellin export in the absence of FliS chaperone.

Mol. Microbiol. 102(3):405-416  2016 PMID:27461872 DOI:10.1111/mmi.13469

Takekawa, N., Terahara, N., Kato, T., Gohara, M., Mayanagi, K., Hijikata, A., Onoue, Y., Kojima, S., Shirai, T., Namba, K. & Homma, M.

The terameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium.

Sci Rep 6:31526  2016 PMID:27531865 DOI:10.1038/srep31526

Kawakita, Y., Kinoshita, M., Furukawa, Y., Tulum, I., Katayama, E., Namba, K. & Miyata, M.

Structural study of MPN387, an essential protein for gliding motility of a human pathogenic bacterium, Mycoplasma pneumonia.

J. Bacteriol. 198:2352-2359  2016 PMID:27325681 DOI:10.1128/JB.00160-16

Matsunami, H., Yoon, Y.-H., Meshcheryakov, V.A., Namba, K. & Samatey, F.A.

Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica.

Sci Rep 6:27399  2016 PMID:27273476 DOI:10.10.8/srep27399

Kinoshita, M., Nakanishi, Y., Furukawa, Y., Namba, K., Imada, K. & Minamino, T.

Rearrangements of α-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.

Mol. Microbiol. 101:656-670  2016 PMID:27178222 DOI:10.1111/mmi.13415

Kawamoto, A., Matsuo, L., Kato, T., Yamamoto, H., Namba, K., Miyata, M.

Periodicity in attachment organelle revealed by electron cryotomography suggests conformational changes in gliding mechanism of Mycoplasma pneumoniae.

mBio 7(2):e00243-16  2016 PMID:27073090 DOI:10.1128/mBio.00243-16

Imada, K., Minamino, T., Uchida, Y., Kinoshita, M., Namba, K.

Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.

Proc. Natl. Acad. Sci. U. S. A. 113(13):3633-3638  2016 PMID:26984495 DOI:10.1073/pnas

Baker, M. A. B., Hynson, R. M. G., Ganuelas, L. A., Mohammadi, N. S., Liew, C. W., Rey, A. A., Duff, A. P., Whitten, A. E., Jeffries, C. M., Delalez, N. J., Morimoto, Y. V., Stock, D., Armitage, J. P., Turberfield, A. J., Namba, K., Berry, R. M. & Lee, L. K.

Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor.

Nat. Struct. Mol. Biol. 23:197-203  2016 PMID:26854663 DOI:10.1073/pnas

Minamino, T., Morimoto, Y.V., Hara, N., Aldridge, P.D. , Namba, K.

The bacterial flagellar type III export gate complex is a dual fuel engine that can use both H+ and Na+ for flagellar protein export.

PLoS Pathog. 4;12(3):e1005495  2016 PMID:26943926 DOI:10.1371/journal.ppat.1005495

Minamino, T., Kinoshita, M., Inoue, Y., Morimoto, Y.V., Ihara, K., Koya, S., Hara, N., Nishioka, N., Kojima, S., Homma, M. & Namba, K.

FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.

MicrobiologyOpen 5(3):424-35  2016 PMID:26916245 DOI:10.1002/mbo3.340

Komatsu, H., Hayashi, F., Sasa, M., Shikata, K., Yamaguchi, S., Namba, K. & Oosawa, K.

Genetic analysis of revertants isolated from the rod-fragile fliF mutant of Salmonella.

Biophysics and Physicobiology 13:13-25  2016 PMID:27924254 DOI:10.2142/biophysico.13.0_13

NEWS

連絡先

〒565-0871 大阪府吹田市山田丘1-3
大阪大学大学院生命機能研究科 ナノバイオロジー棟6階 日本電子YOKOGUSHI協働研究所
TEL: 06-6879-4625
E-mail: keiichi[at]fbs.osaka-u.ac.jp(難波 啓一 特任教授)

  • ※メールアドレスの[at]は@に変換してください

PAGETOP