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FBSコロキウム 313回パターン形成研究室

講演

An artificial mutation introduced in the mechanosensitive cation channel Piezo1 causes morphological abnormality in zebrafish vertebral bone

ラムリ[近藤研究室・D4/D5]

日時 2022年11月10日(木)12:15〜13:00
場所 Zoomでのオンラインセミナーとなります:参加に必要なミーティングリンク、ID、パスワードは事前に、関係者へメールにてご連絡致します。
言語 英語
世話人

渡邉 正勝 准教授
E-mail:watanabe-m[at]fbs.osaka-u.ac.jp
TEL:06-6879-7997

An artificial mutation introduced in the mechanosensitive cation channel Piezo1 causes morphological abnormality in zebrafish vertebral bone

Many studies have found that mechanical forces can affect developmental processes, especially in bone development. On the cellular level, these stimuli are sensed by a specific ion channel called the Piezo channel. When physical forces come, the piezo channel will be activated, allowing cation ions such as calcium to move into the cell. This calcium ion will increase the membrane potential of cells as well as activate several signaling pathways which affect the expression of particular genes related to gene formation. In fact, it has been demonstrated that conditional knock-out of piezo1 channel in mice displays osteoporosis phenotype with lower bone mass, making Piezo1 a potential target for osteoporosis drugs. Interestingly, recent reports have found a correlation between piezo1 and scoliosis disease in humans. However, the molecular mechanism of this process has been still largely unknown because of the difficulty in manipulating the ion channel function artificially in vivo. To analyse in detail, I artificially disrupted piezo1 channel gene using CRISPR/Cas9 system in zebrafish. Here I will present the results of the analysis of Piezo-KO zebrafish.

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