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  4. Structures of a FtsZ single protofilament and a double-helical tube in complex with a monobody

Structures of a FtsZ single protofilament and a double-helical tube in complex with a monobody

Journal Nat. Commun. 14:4073 (2023)
Title Structures of a FtsZ single protofilament and a double-helical tube in complex with a monobody
Laboratory JEOL YOKOGUSHI Research Alliance Laboratories〈SA Prof. NAMBA Keiichi〉
Abstract

FtsZ polymerizes into protofilaments to form the Z-ring that acts as a scaffold for accessory proteins during cell division. Structures of FtsZ have been previously solved, but detailed mechanistic insights are lacking. Here, we determine the cryoEM structure of a single protofilament of FtsZ from Klebsiella pneumoniae (KpFtsZ) in a polymerization-preferred conformation. We also develop a monobody (Mb) that binds to KpFtsZ and FtsZ from Escherichia coli without affecting their GTPase activity. Crystal structures of the FtsZ–Mb complexes reveal the Mb binding mode, while addition of Mb in vivo inhibits cell division. A cryoEM structure of a double-helical tube of KpFtsZ–Mb at 2.7 Å resolution shows two parallel protofilaments. Our present study highlights the physiological roles of the conformational changes of FtsZ in treadmilling that regulate cell division.

Authors

Junso Fujita (1, 2, 3), Hiroshi Amesaka (4), Takuya Yoshizawa (5), Kota Hibino (5), Natsuki Kamimura (5), Natsuko Kuroda (5), Takamoto Konishi (5), Yuki Kato (5), Mizuho Hara (4), Tsuyoshi Inoue (3, 6, 7), Keiichi Namba (1, 2, 8), Shun-Ichi Tanaka (4), Hiroyoshi Matsumura (5)


  1. Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  2. JEOL YOKOGUSHI Research Alliance Laboratories, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  3. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  4. Graduate School of Life and Environmental Science, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, 606-8522, Japan.
  5. Department of Biotechnology, College of Life Sciences, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, 525-8577, Japan.
  6. Open and Transdisciplinary Research Initiatives, Osaka University, 2-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  7. dotAqua Inc., 2-1 Yamadaoka, Suita, Osaka, Japan.
  8. RIKEN Center for Biosystems Dynamics Research and SPring-8 Center, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
PubMed 37429870

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