Biomolecular Networks Laboratories
Laboratory of Medical Biochemistry



Keywords:
Drug discovery, Treatment of diseases, Protein biochemistry, Single cell transcriptome
Developing therapeutics that will change the course of history
Based on the achievements of identifying important factors using various assay systems, we are conducting research utilizing creative protein biochemical techniques. Based on the close relationship between research and clinical laboratories, we have found new drug discovery targets and are promoting first-in-class drug breakthroughs. Currently, the development of eight kinds of drug discovery candidates, such as drugs in the target identification stage, drugs currently being screened, drugs in the compound development stage, in vivo POC stage, and clinical trial stage; etc. are being pursued in parallel. We focus on specific proteins by using advanced antibody production technology, artificial amino acid introduction protein synthesis, an original pretreated mass spectrometer, structural analyses; etc. We are collaborating with a number of pharmaceutical companies as well. Using our abundant research funds, we are seriously working on drug discovery development that targets cardiovascular diseases, psychiatric disorders, metabolic diseases, and cancer. The drugs we produce will alter the course of current medical understanding. Come and join our vision.

Identification of Regulatory Proteins for ATP Production Using Sensitive in vivo ATP Production Assay
Members
TAKASHIMA Seiji (Professor) | takasima[at]cardiology.med.osaka-u.ac.jp |
---|---|
MATSUOKA Ken (Associate Professor) | kmatsuoka[at]medbio.med.osaka-u.ac.jp |
KATO Hisakazu (Assistant Professor, Graduate School of Medicine) | katohisa[at]medbio.med.osaka-u.ac.jp |
TAKADA Eri (Technical Staff, Graduate School of Medicine) | |
KISHIMOTO Mayumi (Technical Staff, Graduate School of Medicine) | |
You could probably reach more information of individual researchers by Research Map and researcher's search of Osaka-U.
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Q&A
- What is your hot research topic?
- The treatment of mitochondrial diseases through the direct activation of oxidative phosphorylation enzymes.
- The treatment of heart failure and skeletal muscle diseases directly with sarcomere activators.
- Drug development using a multi-screening system concerning the life span of protein.
- Elucidation of gene expression control mechanism and gene therapy
- What is your breakthrough or research progress in the last 5 years?
- Increase in new drug discovery pipeline and progress in each pipeline
- What kind of background do your lab members have?
- Half of both instructors and students are composed of Medical Doctor.
- Do you collaborate with other institutions and universities?
- Joint research is being conducted with academia and the business sectors
- What kind of careers do your Lab's alumni go on to?
- Doctors, company researchers.
- How do you develop your research?
- To produce first-in-class drug development.
Research Highlights
Publications (Research Articles, Reviews, Books)
2020
Impact of functional studies on exome sequence variant interpretation in early-onset cardiac conduction system diseases.
Cardiovasc. Res. cvaa010 2020 (PMID:31977013 DOI:10.1093/cvr/cvaa010)
In vivo real-time ATP imaging in zebrafish hearts reveals G0s2 induces ischemic tolerance.
Faseb J. 34(2):2041-2054 2020 (PMID:31916304 DOI:10.1096/fj.201901686R)
Identification of transmembrane protein 168 mutation in familial Brugada syndrome.
Faseb J. 34(5):6399-6417 2020 (PMID:32175648 DOI:10.1096/fj.201902991R)
Higd1a improves respiratory function in the models of mitochondrial disorder
Faseb J. 34:1859-1871 2020 (PMID:31914602 DOI:10.1096/fj.201800389R)
2019
Direct Sarcomere Modulators Are Promising New Treatments for Cardiomyopathies
Int. J. Mol. Sci. 21(1):226 2019 (PMID:31905684 DOI:10.3390/ijms21010226)
Endogenously released adenosine causes pulmonary vasodilation during the acute phase of pulmonary embolization in dogs
Int J Cardiol Heart Vasc 24:100396 2019 (PMID:31334333 DOI:10.1016/j.ijcha.2019.100396)
AST-120, an Adsorbent of Uremic Toxins, Improves the Pathophysiology of Heart Failure in Conscious Dogs.
Cardiovasc. Drugs Ther. 33(3):277-286 2019 (PMID:30903544 DOI:10.1007/s10557-019-06875-z)
Mouse skeletal muscle creatine chemical exchange saturation transfer (CrCEST) imaging at 11.7T MRI.
J. Magn. Reson. Imaging 51(2):563-570 2019 (PMID:31228359 DOI:10.1002/jmri.26844)
A molecular triage process mediated by RING finger protein 126 and BCL2-associated athanogene 6 regulates degradation of G0/G1 switch gene 2.
J. Biol. Chem. 294(40):14562-14573 2019 (PMID:31371451 DOI:10.1074/jbc.RA119.008544)
Mutant KCNJ3 and KCNJ5 Potassium Channels as Novel Molecular Targets in Bradyarrhythmias and Atrial Fibrillation.
Circulation 139(18):2157-2169 2019 (PMID:30764634 DOI:10.1161/CIRCULATIONAHA.118.036761)
Detection of increased intracerebral lactate in a mouse model of Leigh syndrome using proton MR spectroscopy.
Magn. Reson. Imaging 58:38-43 2019 (PMID:30668983 DOI:10.1016/j.mri.2019.01.010)
Impact of cardiac myosin light chain kinase gene mutation on development of dilated cardiomyopathy.
ESC Heart Fail. 6(2):406-415 2019 (PMID:30690923 DOI:10.1002/ehf2.12410)
2018
Anti-HB-EGF Antibody-Mediated Delivery of siRNA to Atherosclerotic Lesions in Mice.
Int. Heart J. 59(6):1425-1431 2018 (PMID:30393262 DOI:10.1536/ihj.17-644)
Fibrin Glue-aided, Instant Epicardial Placement Enhances the Efficacy of Mesenchymal Stromal Cell-Based Therapy for Heart Failure.
Sci Rep 21;8(1):9448 2018 (PMID:29930312 DOI:10.1038/s41598-018-27881-5)
Protein carbamylation exacerbates vascular calcification.
Kidney Int. 94(1):72-90 2018 (PMID:29716796 DOI:10.1016/j.kint.2018.01.033)
Genetic basis of cardiomyopathy and the genotypes involved in prognosis and left ventricular reverse remodeling.
Sci Rep 8(1):1998 2018 (PMID:29386531 DOI:10.1038/s41598-018-20114-9)
2017
Slc3a2 Mediates Branched-Chain Amino-Acid-Dependent Maintenance of Regulatory T Cells.
Cell Reports 21(7):1824-1838 2017 (PMID:29141216 DOI:10.1016/j.celrep.2017.10.082)
Novel Synthesized Radical-Containing Nanoparticles Limit Infarct Size Following Ischemia and Reperfusion in Canine Hearts.
Cardiovasc. Drugs Ther. 31(5-6):501-510 2017 (PMID:29101507 DOI:10.1007/s10557-017-6758-6)
Heat Failure Phenotypes Induced by Knockdown of DAPIT in Zebrafish: A New Insight into Mechanism of Dilated Cardiomyopathy.
Auris Nasus Larynx 7(1):17417 2017 (PMID:29234032 DOI:10.1038/s41598-017-17572-y)
Alternative exon skipping biases substrate preference of the deubiquitylase USP15 for mysterin/RNF213, the moyamoya disease susceptibility factor.
Autophagy 7:44293 2017 (PMID:28276505 DOI:10.1038/srep44293)
Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes.
Sci Rep 7(1):9363 2017 (PMID:28839205 DOI:10.1038/s41598-017-09716-x)
Radiation-induced HFpEF model as a potential tool for the exploration of novel therapeutic targets.
Am. J. Physiol.-Heart Circul. Physiol. 313(2):H323-H325 2017 (PMID:28626077 DOI:10.1152/ajpheart.00307.2017)
2016
Adenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines.
Sci Rep 6:36946 2016 (PMID:27833131 DOI:10.1038/srep36946)
Cell Size Critically Determines Initial Retention of Bone Marrow Mononuclear Cells in the Heart after Intracoronary Injection: Evidence from a Rat Model.
PLoS One 11(7):e0158232 2016 (PMID:27380410 DOI:10.1371/journal.pone.0158232)
Alternatively activated macrophages determine repair of the infarcted adult murine heart.
J. Clin. Invest. 126(6):2151-66 2016 (PMID:27140396 DOI:10.1172/JCI85782)
A specific single-stranded DNA induces a distinct conformational changecrossmark in the nucleoid-associated protein HU
Biochemistry and Biophysics Reports 8:318-324 2016 (PMID:28955971 DOI:10.1016/j.bbrep.2016.09.014)
Allogeneic Mesenchymal Stromal Cells Transplanted Onto the Heart Surface Achieve Therapeutic Myocardial Repair Despite Immunologic Responses in Rats.
J. Am. Heart Assoc. 5(2):e002815 2016 (PMID:26896478 DOI:10.1161/JAHA.115.002815)
Targeted Therapy for Acute Autoimmune Myocarditis with Nano-Sized Liposomal FK506 in Rats.
PLoS One 1(8):e0160944 2016 (PMID:27501378 DOI:10.1371/journal.pone.0160944)
Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy.
J. Am. Heart Assoc. 5(7):e003521 2016 (PMID:27385424 DOI:10.1161/JAHA.116.003521)
Btg2 is a Negative Regulator of Cardiomyocyte Hypertrophy through a Decrease in Cytosolic RNA.
Sci Rep 6:28592 2016 (PMID:27346836 DOI:10.1038/srep28592)
A Development of Nucleic Chromatin Measurements as a New Prognostic Marker for Severe Chronic Heart Failure.
PLoS One 11(2):e0148209 2016 (PMID:26845691 DOI:10.1371/journal.pone.0148209)
2015
Single-Cell Expression Profiling Reveals a Dynamic State of Cardiac Precursor Cells in the Early Mouse Embryo.
PLoS One 10(10):e0140831 2015 (PMID:26469858 DOI:10.1371/journal.pone.0140831)
Our ideal candidate (as a graduate student)
We are looking for a highly motivated person to work on our research topics as our lab member. Our lab welcomes the person who loves taking care of creatures, hand working and handcraft too. Any kind of background (such as your expertise or major) is available.
Contact
Laboratory of Medical Biochemistry, Graduate School of Frontier Biosciences, Osaka University,
2-2 Yamadaoka, Suita, Osaka 565-0871 Japan.
TEL:+81-6-6879-3492
E-mail: takasima[at]cardiology.med.osaka-u.ac.jp (Prof. Seiji Takashima)
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