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Integrated Biology Laboratories

Laboratory of Pattern Formation

Prof. Shigeru Kondo Prof. Shigeru Kondo

Keyword:

Skin pattern of fish, Reaction diffusion, Corners of beetles, Formation of fin bones, 3D structure

Our aim is to clarify the mechanisms generating the spatial pattern and the shape of organisms

Functions of the organs and tissues in our body depend on their shape. How are such shapes are formed and maintained? One of the answers to this question is "gene(s)". It is true. However, it is impossible to explain the building of the structure that is much larger than the size of cells only by the function of genes because genes only functions in cells. An alternative answer is “rules of physics”. We have proved that the skin pattern of fish is formed by a kind of wave (Turing wave) derived from the interaction between two kinds of pigment cells. Recently, we started projects to clarify the mechanisms generating the 3D structure of the bones of fish and the exoskeletons of insects. If you want to know the answer, please knock the door of our laboratory. You will see something unexpected and fascinating.

Members

Shigeru Kondo (Professor) skondo[at]fbs.osaka-u.ac.jp
Masakatsu Watanabe (Associate Professor) watanabe-m[at]fbs.osaka-u.ac.jp
Toshihiro Aramaki (Assistant Professor) taramaki[at]fbs.osaka-u.ac.jp
Junpei Koroda (Assistant Professor)  
Seita Miyazawa(Specially Appointed Researcher) seita[at]fbs.osaka-u.ac.jp
Keiko Takeda (Secretary)  
Mana Kadota (Technical Staff)  
Nozomi Takada (Technical Staff)  

You could probably reach more information of individual researchers by Research Map and researcher's search of Osaka-U.

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Q&A

What is your hot research topic?
Regarding the research of the bone morphogenesis, the relationship between the collagen crystals and the cells that manipulate them is interesting. The cells assemble pillars (collagen crystals) to make fin bones in the way similar to the workers on a construction site with a division of labor. Also interesting is the mechanism of the sudden emergence of complex and huge structures, such as beetles and horned horns. Insects create a huge structure in a compactly folded state, and then inflate it at a stretch, allowing sudden morphological changes. we named this method a reversed Origami method.
What is your breakthrough or research progress in the last 5 years?
The principle of folding the corners of beetles is mostly understood. The three-dimensional structure of the horns is encrypted into a two-dimensional pattern of the furrows on the surface of the imaginal disc of the horn, and various shapes of horns can appear by changing the furrow pattern.
What kind of background do your lab members have?
3D morphogenesis of vertebrates is also very fascinating. The functions of cells that manipulate collagen crystals to construct the fin bones is unexpectedly dynamic, and we are surprised every day by the depth of morphogenesis.
Do you collaborate with institutions outside of Osaka Univ.?
We often collaborate with specialists in physical simulation and mathematical models. The basic stance of our laboratory is “proving by experiment”. Therefore, most members in our laboratory conduct experiment-based research. When the phenomenon turned out to be very complex, mathematical processing such as simulation is often required to understand. In such cases, we ask specialists to join us and do the simulation together. We don't leave all simulations to the experts. We believe that it is necessary for those involved in research to understand both experiments and research.
What kind of careers do your Lab's alumni go on to?
Those who have obtained a doctorate are mostly working in academia, and are working as researchers at universities, RIKEN, and life science ventures. In addition, one individual became a curator of an aquarium using her experience in fish pattern research. Most of the Master's graduates have joined different companies.
How do you develop your research?
Our research is similar to a treasure hunt adventure. I would like to find a "treasure" such as a folding method that can produce all external forms of insects, the principle of creating 3D forms of arbitrary bones, and the principle of determining the size of bones.

Research Highlights

Publications (Research Articles, Reviews, Books)

2020

Burendei, B., Shinozaki, R., Watanabe, M., Terada, T., Tani, K., Fujiyoshi, Y., Oshima, A.

Cryo-EM structures of undocked innexin-6 hemichannels in phospholipids

Sci. Adv. 6(7):eaax3157  2020 PMID:32095518 DOI:10.1126/sciadv.aax3157

Toshihiro Aramaki, Shigeru Kondo

Method for disarranging the pigment pattern of zebrafish by optogenetics.

Dev. Biol. 460(1):12-19  2020 PMID:30578760 DOI:10.1016/j.ydbio.2018.12.019

2019

Usui, Y., Aramaki, T., Kondo, S., Watanabe, M.

The minimal gap-junction network among melanophores and xanthophores required for stripe-pattern formation in zebrafish

Development 146(22)  2019 PMID:31666235 DOI:10.1242/dev.18106

Sakashita M, Sato M, Kondo S

Comparative morphological examination of vertebral bodies of teleost fish using high-resolution micro-CT scans.

J. Morphol. 280(6):778-795  2019 PMID:30945336 DOI:10.1002/jmor.20983

Denis, J. F., Diagbouga, M. R., Molica, F., Hautefort, A., Linnerz, T., Watanabe, M., Lemeille, S., Bertrand, J. Y., Kwak, B. R.

KLF4-Induced Connexin40 Expression Contributes to Arterial Endothelial Quiescence.

Front. Physiol. 0.472222222  2019 PMID:30809154 DOI:10.3389/fphys.2019.00080

2018

Marc Chanson, Masakatsu Watanabe, Erin M. O’Shaughnessy, Alice Zoso, Patricia E. Martin

Connexin Communication Compartments and Wound Repair in Epithelial Tissue

Int. J. Mol. Sci. 19(5):1354  2018 PMID:29751558 DOI:10.3390/ijms19051354

Risa Sawada, Toshihiro Aramaki, Shigeru Kondo

Flexibility of pigment cell behavior permits the robustness of skin pattern formation

Genes Cells 23(7):537-545  2018 PMID:29797484 DOI:10.1111/gtc.12596

Adachi, H., Matsuda, K., Niimi, T., Inoue, Y., Kondo, S., Gotoh, H.

Anisotropy of cell division and epithelial sheet bending via apical constriction shape the complex folding pattern of beetle horn primordia

Mech. Dev. 152:32-37  2018 PMID:29920372 DOI:10.1016/j.mod.2018.06.003

Yuu Usui, Shigeru Kondo, Masakatsu Watanabe

Melanophore multinucleation pathways in zebrafish

Dev. Growth Diff. 60:454-459  2018 PMID:30088265 DOI:10.1111/dgd.12564

Ryo Mizuuchi, Hiroshi Kawase, Hirofumi Shin, Daisuke Iwai, Shigeru Kondo

Simple rules for construction of a geometric nest structure by pufferfish

Sci Rep 8(1):12366  2018 PMID:30120331 DOI:10.1038/s41598-018-30857-0

2017

Keisuke Matsuda, Hiroki Gotoh, Yuki Tajika, Takamichi Sushida, Hitoshi Aonuma, Teruyuki Niimi, Masakazu Akiyama, Yasuhiro Inoue, Shigeru Kondo

Complex furrows in a 2D epithelial sheet code the 3D structure of a beetle horn

Sci Rep 7(1):13939  2017 PMID:29066748 DOI:10.1038/s41598-017-14170-w

Masakatsu Watanabe

Gap Junction in the Teleost Fish Lineage: Duplicated Connexins May Contribute to Skin Pattern Formation and Body Shape Determination

Frontiers in Cell Dev. Biol, Cell Adhesion and Migration   2017 PMID:28271062 DOI:10.3389/fcell.2017.00013

2016

Shigeru Kondo

An updated kernel-based Turing model for studying the mechanisms of biological pattern formation

J. Theor. Biol. 414:120-127  2016 PMID:27838459 DOI:10.1016/j.jtbi.2016.11.003

Watanabe, M., Kondo, S.

Genetics of body shape: Connexin43 is the key to two zebrafish mutants with shorter backbones and fins

Atlas of Science   2016

Shinya Inoue, Shigeru Kondo

Suture pattern formation in ammonites and the unknown rear mantle structure

Sci Rep 6:33689  2016 PMID:27640361 DOI:10.1038/srep33689

Akihiro Misu, Hiroaki Yamanaka, Toshihiro Aramaki, Shigeru Kondo, I. Martha Skerrett, M. Kathryn Iovine, Masakatsu Watanabe

Two different functions of Connexin43 confer two different bone phenotypes in zebrafish

J. Biol. Chem. 291(24):12601-11  2016 PMID:27129238 DOI:10.1074/jbc.M116.720110

Ideal Candidate (To Graduate Student)

We are looking for a highly motivated person to work on our research topics as our lab member. Our lab welcomes the person who loves taking care of creatures, hand working and handcraft too. Any kind of background (such as your expertise or major) is available.

Contact

Laboratory of Pattern Formation, Graduate School of Frontier Biosciences, Osaka University,
1-3 Yamadaoka, Suita, Osaka 565-0871 Japan.

Tel:+81-6-6879-7976

E-mail: skondo[at]fbs.osaka-u.ac.jp (Prof. Shigeru Kondo)

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