Nanobiology Laboratories
Laboratory of Nano-Biophysics
Prof. ISHIJIMA Akihiko
Keywords:
Single molecule measurement, Bacteria, Bacterial flagellar motor, Chemotaxis
Clarifying the working mechanisms of biomolecules
In biological systems, nanometer-scale macromolecules are key players in various physiological functions, including movement and signal transduction. However, these mechanisms have not been understood well. In order to understand the fundamental principles of the working mechanism of biomolecules, in my laboratory we are developing single molecule measurement and imaging systems that are capable of measuring the movements of biomolecules at the nanometer and Pico Newton scales. Our goal is to clarify the working mechanisms of biomolecules, such as the actomyosin motor, bacterial flagellar motor, and signal transduction system using the newest equipment.
Bacterial flagellar motor. Rotary motor embedded in membrane rotates using flow of ions.
Members
| ISHIJIMA Akihiko (Professor) | ishijima.fbs[at]osaka-u.ac.jp |
|---|---|
| FUKUOKA Hajime (Associate Professor) | fukuoka.hajime.fbs[at]osaka-u.ac.jp |
| UCHIDA Yumiko (Assistant Professor) | uchida.yumiko.fbs[at]osaka-u.ac.jp |
| Maeda Rie (Secretary) |
You could probably reach more information of individual researchers by Research Map and researcher's search of Osaka-U.
- ※Change [at] to @
Q&A
- What is your hot research topic?
- To understand the mechanism of the bacterial flagellar motor and signal transduction of chemotaxis in E. coli.
- Have you had any breakthroughs or significant research progress in the last 5 years?
- What kind of background do your lab members have?
- Most of the graduate students are from the School of Engineering Science. Others are from outside the university.
- Do you collaborate with other institutions and universities?
- We are in collaboration with Sandy Parkinson’s Lab at the University of Utah regarding chemotaxis. We are also collaborating with researchers at Oxford University.
- What kind of careers do your Lab's alumni go on to?
- Academia researchers, pharmaceutical company researchers, venture company entrepreneurs, and others.
- How do you develop your research?
- Although Escherichia coli is a bacterium, it has all the molecules and functions necessary to maintain a living system. Many of the proteins related to chemotaxis and motor functions have been identified in E. coli. Therefore, we use E. coli cells to reveal how, when, how many of, and with which other molecules these proteins function in a single cell.
Research Highlights
Publications (Research Articles, Reviews, Books)
2024
CheB localizes to polar receptor arrays during repellent adaptation
Science Advances 10(38):eadp5636 2024 (PMID:39303042 DOI:10.1126/sciadv.adp5636)
2022
The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an?Escherichia coli?Cell
Journal of Bacteriology 204(12):e00278-22 2022 (PMID:36448786 DOI:10.1128/jb.00278-22)
2020
Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli
Biomolecules 10(11):1544 2020 (PMID:33198296 DOI:10.3390/biom10111544)
2017
The Bacterial Flagellum -Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis-
Methods in Molecular Biology 1593:215-226 2017 (PMID:28389957 DOI:10.1007/978-1-4939-6927-2_17)
2016
Local heating of molecular motors using single carbon nanotubes
Biophysical Reviews 8(1):25-32 2016 (PMID:28510142 DOI:10.1007/s12551-015-0185-3)
Our ideal candidate (as a graduate student)
We are looking for highly motivated people to work on our research topics. Our lab welcomes people who love living things, enjoy delicate work with their hands, and creating things. We welcome everyone with any background, expertise, or majors.
Contact
Laboratory of Nano-Biophysics, Graduate School of Frontier Biosciences, Osaka University,
1-3 Yamadaoka, Suita, Osaka 565-0871 Japan.
TEL: +81-6-6876-4650
email: ishijima.fbs[at]osaka-u.ac.jp (Prof. ISHIJIMA Akihiko)
- ※Change [at] to @
