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Nanobiology Laboratories

Laboratory of Nano-Biophysics

Prof. ISHIJIMA Akihiko Prof. ISHIJIMA Akihiko

Keywords:

Single molecule measurement, Bacteria, Bacterial flagellar motor, Chemotaxis

Clarifying the working mechanisms of biomolecules

In biological systems, nanometer-scale macromolecules are key players in various physiological functions, including movement and signal transduction. However, these mechanisms have not been understood well. In order to understand the fundamental principles of the working mechanism of biomolecules, in my laboratory we are developing single molecule measurement and imaging systems that are capable of measuring the movements of biomolecules at the nanometer and Pico Newton scales. Our goal is to clarify the working mechanisms of biomolecules, such as the actomyosin motor, bacterial flagellar motor, and signal transduction system using the newest equipment.

 

Bacterial flagellar motor. Rotary motor embedded in membrane rotates using flow of ions.

Members

ISHIJIMA Akihiko (Professor) ishijima.fbs[at]osaka-u.ac.jp
FUKUOKA Hajime (Associate Professor) fukuoka.hajime.fbs[at]osaka-u.ac.jp
UCHIDA Yumiko (Assistant Professor) uchida.yumiko.fbs[at]osaka-u.ac.jp
Maeda Rie (Secretary)  

You could probably reach more information of individual researchers by Research Map and researcher's search of Osaka-U.

 
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Q&A

What is your hot research topic?
To understand the mechanism of the bacterial flagellar motor and signal transduction of chemotaxis in E. coli.
Have you had any breakthroughs or significant research progress in the last 5 years?
What kind of background do your lab members have?
Most of the graduate students are from the School of Engineering Science. Others are from outside the university.
Do you collaborate with other institutions and universities?
We are in collaboration with Sandy Parkinson’s Lab at the University of Utah regarding chemotaxis. We are also collaborating with researchers at Oxford University.
What kind of careers do your Lab's alumni go on to?
Academia researchers, pharmaceutical company researchers, venture company entrepreneurs, and others.
How do you develop your research?
Although Escherichia coli is a bacterium, it has all the molecules and functions necessary to maintain a living system. Many of the proteins related to chemotaxis and motor functions have been identified in E. coli. Therefore, we use E. coli cells to reveal how, when, how many of, and with which other molecules these proteins function in a single cell.

Research Highlights

Publications (Research Articles, Reviews, Books)

2024

Hajime Fukuoka, Keisuke Nishitani, Taiga Deguchi, Taketo Oshima, Yumiko Uchida, Tatsuki Hamamoto, Yong-Suk Che, and?Akihiko Ishijima

CheB localizes to polar receptor arrays during repellent adaptation

Science Advances 10(38):eadp5636  2024 PMID:39303042 DOI:10.1126/sciadv.adp5636

2022

Yumiko Uchida, Tatsuki Hamamoto, Yong-Suk Che, Hiroto Takahashi, John S. Parkinson,?Akihiko Ishijima, Hajime Fukuoka

The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an?Escherichia coli?Cell

Journal of Bacteriology 204(12):e00278-22  2022 PMID:36448786 DOI:10.1128/jb.00278-22

2020

Yong-Suk Che, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, Tatsuki Hamamoto, Akihiko Ishijima, Hajime Fukuoka

Fluctuations in Intracellular CheY-P Concentration Coordinate Reversals of Flagellar Motors in E. coli

Biomolecules 10(11):1544  2020 PMID:33198296 DOI:10.3390/biom10111544

2017

Hajime Fukuoka

The Bacterial Flagellum -Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis-

Methods in Molecular Biology 1593:215-226  2017 PMID:28389957 DOI:10.1007/978-1-4939-6927-2_17

2016

Yuichi Inoue, Akihiko Ishijima

Local heating of molecular motors using single carbon nanotubes

Biophysical Reviews 8(1):25-32  2016 PMID:28510142 DOI:10.1007/s12551-015-0185-3

Our ideal candidate (as a graduate student)

We are looking for highly motivated people to work on our research topics. Our lab welcomes people who love living things, enjoy delicate work with their hands, and creating things. We welcome everyone with any background, expertise, or majors.

Contact

Laboratory of Nano-Biophysics, Graduate School of Frontier Biosciences, Osaka University,
1-3 Yamadaoka, Suita, Osaka 565-0871 Japan.

TEL: +81-6-6876-4650

email: ishijima.fbs[at]osaka-u.ac.jp (Prof. ISHIJIMA Akihiko)

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