FBS Colloquia No.400Laboratory of Immunology and Cell Biology

Development of highly multiplex 3D imaging method Ce3D-IBEX and the identification of vagus-associated lymphoid tissue in the lung

Human tissues consist of over 200 types of cells forming complex three-dimensional (3D) structures, and many biological phenomena highly rely on these 3D tissue structures. Therefore, to understand tissue biology and immunology, it is essential to understand how individual cells are distributed within the three-dimensional structure of the tissue to shape their optimal output. To address this, I developed a novel 3D multiplex imaging technique, Ce3D-IBEX. Ce3D-IBEX enables the detection of expression levels for over 30 proteins within 3D tissue and is currently the only method capable of simultaneously visualizing multiple subsets of immune cells and tissue structures. In this presentation, I will showcase the Ce3D-IBEX technique and discuss the biology of novel lymphoid structures in the lung associated with the vagus nerve.