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FBS Colloquia No.377Physiological Laboratory

Seminar or Lecture

Real-time measurements of ion channel activities and calcium concentration changes in nano-biostructures olfactory cilia

Hiroko Takeuchi [Associate Professor, Physiological Laboratory]

Date and Time 21 Jan. 2025 (Tue), 12:15~13:00
Place 2F Seminar Room, BioSystems Building
Language Japanese
Contact

Hiroko Takeuchi (Associate Professor)
E-mail: takeuchi.hiroko.fbs[at]osaka-u.ac.jp
TEL: 06-6879-7996

Real-time measurements of ion channel activities and calcium concentration changes in nano-biostructures olfactory cilia

Our sense of smell begins with the olfactory receptor cells (ORCs), which receive odor information. Chemical information is converted into electrical signals by ion channels on the cilia, and the signal is transmitted to the brain. To understand this system under physiological condition, it is necessary to study the odorant response of the fresh native ORCs and molecular movement in living cilia. However, nanoscale microstructures of cilia have made it difficult to handle native and healthy olfactory cilia for physiological investigations. To overcome such difficulty, we have developed new experimental techniques to record the electrical excitation through the transduction channels of living cilia while simultaneously visualizing the movement of Ca2+ inside the cilia.We have demonstrated that Ca2+ plays a dual role in olfactory cilia; signal amplification and olfactory adaptation, both are required for our sense of smell. Our data show that two functions are well segregated and controlled. In this colloquium we will introduce our combined devices and findings from cell data for understanding the function of living cilia.

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