FBS Colloquia No.237Building Block Science Joint Research Chair

In vitro cell manipulation by building block science: Construction of LbL-3D tissue for drug development and regenerative medicine

The fabrication of artificial three-dimensional (3D) tissues with similar function to native organ is a greatly challenge for implantable tissues in regenerative medicine, and for in vitro 3D tissue models in drug discovery field. We reported a simple bottom-up approach, termed “cell-accumulation technique”, to develop 3D cellular multilayer (LbL-3D tissue) by using layer-by-layer (LbL) coating technique of extracellular matrix (ECM) proteins onto the cell surfaces. Using this technique, we constructed 3D models of human tissue; skin model, myocardial model, liver model, islet model peritoneal model and oral mucosal model. Especially for skin and myocardial model (LbL-3D Skin and LbL-3D Heart), we are proceeding the international standardization of our developed tissues as alternative systems to animal testing and their application in regenerative medicine. We also focus on “Development of fully automated manufacturing system of LbL-3D tissue” for production with high safety and reproducibility toward practical use