FBS Colloquia No.186JEOL YOKOGUSHI Research Aliance Laboratories
Seminar or Lecture |
The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM Takayuki Kato [JEOL YOKOGUSHI Research Alliance Laboratories] The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM Tomoko Miyata [JEOL YOKOGUSHI Research Alliance Laboratories] |
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Date and Time | Wednesday, May 30, 2018, 12:15-13:00 |
Place | 2F Seminar room, BioSystems Building |
Contact |
Takayuki Kato |
The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM
X-ray crystallography, NMR, and electron cryomicroscopy (cryoEM) are important techniques for structural analysis of biological macromolecules. Each technique has its own advantages and disadvantages but cryoEM is the most versatile in terms of sample state and condition and can be used in a far wider range of specimens than the other two. CryoEM was not so popular than others until a few years ago because generally attainable resolution was limited to around 10 Å. However, the resolution was dramatically improved by the recent development of direct electron detection camera. That is why macromolecular structures solved by cryoEM image analysis are found in top level journals, such as Nature and Science, in almost every issue. The Nobel Prize in Chemistry was awarded to three pioneers in the cryoEM field because of the present power and usefulness of the technique for life and medical sciences. In this colloquium, we will describe the structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM.
The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM
X-ray crystallography, NMR, and electron cryomicroscopy (cryoEM) are important techniques for structural analysis of biological macromolecules. Each technique has its own advantages and disadvantages but cryoEM is the most versatile in terms of sample state and condition and can be used in a far wider range of specimens than the other two. CryoEM was not so popular than others until a few years ago because generally attainable resolution was limited to around 10 Å. However, the resolution was dramatically improved by the recent development of direct electron detection camera. That is why macromolecular structures solved by cryoEM image analysis are found in top level journals, such as Nature and Science, in almost every issue. The Nobel Prize in Chemistry was awarded to three pioneers in the cryoEM field because of the present power and usefulness of the technique for life and medical sciences. In this colloquium, we will describe the structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM.