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Research seminars CENcycolopedia: The dynamic landscape of kinetochore architecture throughout the cell cycle

Seminar or Lecture
Date and Time 6 Feb. 2025 (Thu), 17:00-18:00
Place 2F Seminar Room, BioSystems Building
Contact

Tatsuo Fukagawa
E-mail: fukagawa.tatsuo.fbs[at]osaka-u.ac.jp
TEL: 06-6879-4428

Abstract

In the first half of the seminar, I will introduce our recent advancements in super-resolution microscopy techniques. Super-resolution microscopy has become an indispensable tool in cell biology, providing unparalleled insights into biological architectures with nanometer precision. This technology offers several advantages, including the simultaneous labeling of multiple target biomolecules with high specificity and simplified sample preparation, making it widely accessible to researchers. We have recently developed 4-fold and 12-fold isotropic and 3D-preserved Expansion Microscopy (4× and 12× 3D-ExM). This innovative method offers a streamlined, single-step process for achieving robust and reproducible 3D isotropic expansion, applicable to both 2D and 3D cell culture models. Using standard confocal microscopy, 12× 3D-ExM a achieves a lateral resolution of <30 nm, enabling the visualization of nanoscale structures such as chromosomes, kinetochores, nuclear pore complexes, and Epstein–Barr virus particles. In the latter half of the seminar, I will focus on the dynamic architecture of kinetochores ensuring faithful chromosome segregation. The kinetochore plays a pivotal role in orchestrating chromosome segregation. It serves as a platform for microtubule assembly, monitors microtubule binding fidelity, and acts as a force coupler during mitosis. Composed of more than 100 distinct proteins, many of which exist in multiple copies, the kinetochore undergoes dynamic compositional changes throughout the cell cycle, adapting to specific stages and conditions. Using advanced quantitative immunofluorescence microscopy, we have systematically mapped the dynamics of kinetochore protein levels across the cell cycle, offering a comprehensive view of the temporal and spatial organization of kinetochore architecture.

References
  • Norman, R., Chen, YC., Recchia M., Loi, J., Rosemarie, Q., Lesko, S., Patel, S., Sherer, NM., Takaku, M., Burkard, M., Suzuki, A.
    One step 4x and 12x 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.
    Journal of Cell Biology, 224 (2): e202407116, 2025.
  • Chen, YC., Kilic, E., Wang, E., Rossman, W., Suzuki, A.
    CENcyclopedia: Dynamic Landscape ofKinetochore Architecture Throughout the Cell Cycle.
    bioRxiv, 2024.12.05.627000; doi: https://doi.org/10.1101/2024.12.05.627000

If you want to speak Dr.Suzuki in person, please let me know. I will arrange the Interview with him.

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