Research seminars Visualization of transcription hubs in living Drosophila embryos
| Seminar or Lecture |
Takashi Fukaya [Associate Professor, Laboratory of Transcriptional Dynamics, Institute for Quantitative BioSciences, The University of Tokyo] |
|---|---|
| Date and Time | 19 Jul. 2023 (Wed), 15:00-16:00 |
| Place | 2F Seminar Room, BioSystems Building |
| Language | Japanese, with slides in English |
| Contact |
Takeo Horie (Professor) E-mail: horie.takeo.fbs[at]osaka-u.ac.jp |
Abstract
The initial step in the transfer of genetic information is a process known as "transcription", where an enzyme called RNA polymerase synthesizes an RNA copy of the DNA sequence. Through past biochemical, structural, and whole-genome sequencing studies, the molecular mechanisms of transcription by RNA polymerase have been elucidated in great detail. However, the temporal dynamics of this process still remains an outstanding question in modern biology. Importantly, from bacteria to humans, emerging evidence suggests that the prevalent feature of gene expression conserved across species is intermittent bursts of de novo transcription, or "transcriptional bursting". However, the molecular mechanism underlying the control of transcriptional bursting still remains a central question in the field. Our lab uses quantitative MS2/PP7 live-imaging and variety of genome engineering methods to study the regulatory mechanism of transcriptional bursting in developing Drosophila embryos. In this seminar, I will discuss “transcription hubs” to explain the temporal and spatial specificity of gene activities during early embryogenesis.
References
Molecular Cell. 83, 1605-1622. 2023.
Molecular Cell. 78, 224-235.2020.
Molecular Cell. 70, 287-296. 2018
Cell. 166, 358–368, 2016
