2025
- Sridhar S, Fukagawa T.
Meiosis: When centromeres choose compromise over conflict.
Curr Biol 35(5):R196-R198. doi: 10.1016/j.cub.2025.01.059.(2025) PubMed - Asakawa H, Nagao K, Fukagawa T, Obuse C, Hiraoka Y, Haraguchi T.
Interaction mapping between nucleoporins in the fission yeast Schizosaccharomyces pombe using mass-spectrometry.
J Biochem 177(4):273-286. doi: 10.1093/jb/mvae095. (2025) PubMed
2024
- Takenoshita Y, Hara M, Nakagawa R, Ariyoshi M, Fukagawa T.
Molecular details and phosphoregulation of the CENP-T-Mis12 complex interaction during mitosis in DT40 cells.
iScience 27(12):111295. doi: 10.1016/j.isci.2024.111295. (2024) PubMed - Kong W, Hara M, Tokunaga Y, Okumura K, Hirano Y, Miao J, Takenoshita Y, Hashimoto M, Sasaki H, Fujimori T, Wakabayashi Y, Fukagawa T.
CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.
Life Sci Alliance 8(1):e202402927. doi: 10.26508/lsa.202402927. (2024) PubMed - Hirano Y, Sato T, Miura A, Kubota Y, Shindo T, Fukase K, Fukagawa T, Kabayama K, Haraguchi T, Hiraoka Y.
Disordered region of nuclear membrane protein Bqt4 recruits phosphatidic acid to the nuclear envelope to maintain its structural integrity
J Biol Chem 1300(7):107430. doi: 10.1016/j.jbc.2024.107430. (2024) PubMed - Sacristan C, Samejima K, Ruiz LA, Deb M, Lambers MLA, Buckle A, Brackley CA, Robertson D, Hori T, Webb S, Kiewisz R, Bepler T, van Kwawegen E, Risteski P, Vukušić K, Tolić IM, Müller-Reichert T, Fukagawa T, Gilbert N, Marenduzzo D, Earnshaw WC, Kops GJPL.
Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin.
Cell 187(12):3006-3023.e26. doi: 10.1016/j.cell.2024.04.014. (2024) PubMed - Cao J, Hori T, Ariyoshi M, Fukagawa T.
Artificial tethering of constitutive centromere-associated network proteins induces CENP-A deposition without Knl2 in DT40 cells.
J Cell Sci 137(2):jcs261883. doi: 10.1242/jcs.261883. (2024) PubMed
2023
- Ariyoshi M, Fukagawa T.
An updated view of the kinetochore architecture.
Trends Genet 39(12):941-953. doi: 10.1016/j.tig.2023.09.003. (2023) PubMed - Nagpal H, Ali-Ahmad A, Hirano Y, Cai W, Halic M, Fukagawa T, Sekulić N, Fierz B.
CENP-A and CENP-B collaborate to create an open centromeric chromatin state.
Nature Communu 14(1):8227. doi: 10.1038/s41467-023-43739-5. (2023) PubMed - Yabushita T, Chinen T, Nishiyama A, Asada S, Shimura R, Isobe T, Yamamoto K, Sato N, Enomoto Y, Tanaka Y, Fukuyama T, Satoh H, Kato K, Saitoh K, Ishikawa T, Soga T, Nannya Y, Fukagawa T, Nakanishi M, Kitagawa D, Kitamura T, Goyama S.
Mitotic perturbation is a key mechanism of action of decitabine in myeloid tumor treatment.
Cell Rep 42(9):113098. doi: 10.1016/j.celrep.2023.113098.(2023) PubMed - Le TK, Hirano Y, Asakawa H, Okamoto K, Fukagawa T, Haraguchi T, Hiraoka Y.
A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.
J Cell Sci 136(19):jcs260930. doi: 10.1242/jcs.260930. (2023) PubMed - Hara M, Ariyoshi M, Sano T, Nozawa R, Shinkai S, Onami S, Jansen L, Hirota T, Fukagawa T.
Centromere/kinetochore is assembled through CENP-C oligomerization.
Mol Cell 83(13):2188-2205.e13. doi: 10.1016/j.molcel.2023.05.023. (2023) PubMed - Hirano Y, Ohno Y, Kubota Y, Fukagawa T, Kihara A, Haraguchi T, Hiraoka Y.
Ceramide synthase homolog Tlc4 maintains nuclear envelope integrity via its Golgi translocation.
J Cell Sci DOI: 10.1242/jcs.260923 PubMed - Huang Z, Xu Z, Bai H, Huang Y, Kang N, Ding X, Liu J, Luo H, Yang C, Chen W, Guo Q, Xue L, Zhang X, Xu L, Chen M, Fu H, Chen Y, Yue Z, Fukagawa T, Liu S, Chang G, Xu L.
Evolutionary analysis of a complete chicken genome.
Proc Natl Acad Sci USA 120(8):e2216641120.(2023) PubMed - Jiang H, Ariyoshi M, Hori T, Watanabe R, Makino F, Namba K, Fukagawa T.
The cryo-EM structure of the CENP-A nucleosome in complex with ggKNL2.
EMBO J e111965. (2023) PubMed - Fukagawa T, Kakutani T
Transgenerational epigenetic control of constitutive heterochromatin, transposons, and centromeres.
Curr Opin Genet Dev 10:862637 (2023) PubMed
2022
- Sridhar S, Fukagawa T.
Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution .
Front Cell Dev Biol 10:862637 (2022) PubMed - Takenoshita Y, Hara M, Fukagawa T
Recruitment of two Ndc80 complexes via the CENP-T pathway is sufficient for kinetochore functions.
Nature Commun 13:851 (2022) PubMed - Haraguchi T, Koujin T, Shindo T, Bilir Ş, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase.
Commun Biol 5:78 (2022) PubMed - Watanabe R, Hirano Y, Hara M, Hiraoka Y, Fukagawa T
Mobility of kinetochore proteins measured by FRAP analysis in living cells.
Chromosome Res 30:43-57. doi: 10.1007/s10577-021-09678-x. (2022) PubMed - Nishimura K, Fukagawa T
A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines.
Methods Mol Biol. 2377:109-122. doi: 10.1007/978-1-0716-1720-5_6. (2022) PubMed
2021
- Chen Q, Zhang M, Pan X, Yuan X, Zhou L, Yan L, Zeng LH, Xu J, Yang B, Zhang L, Huang J, Lu W, Fukagawa T, Wang F, Yan H
Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation.
Cell Rep 36(12):109740. doi: 10.1016/j.celrep.2021.109740. (2021) PubMed - Nishimura K, Fukagawa T
A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines.
Bio Protoc e11(14):e4092. doi: 10.21769/BioProtoc.4092. (2021) PubMed - Uchida KSK, Jo M, Nagasaka K, Takahashi M, Shindo N, Shibata K, Tanaka K, Masumoto H, Fukagawa T, Hirota T
Kinetochore stretching-mediated rapid silencing of the spindle-assembly checkpoint required for failsafe chromosome segregation.
Curr Biol 31(8):1581-1591.e3. doi: 10.1016/j.cub.2021.01.062. (2021) PubMed - Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
Cryo-EM structure of the CENP-A nucleosome in complex with phosphorylated CENP-C.
EMBO J 40:e105671. doi: 10.15252/embj.2020105671. (2021) PubMed - Watanabe R, Hara M, Ariyoshi M, Fukagawa T
CENP-C Phosphorylation by CDK1 in vitro.
Bio Protoc 11(1):e3879. doi: 10.21769/BioProtoc.3879. (2021) PubMed - Sridhar S, Hori T, Nakagawa R, Fukagawa T, Sanyal K
Bridgin connects the outer kinetochore to centromeric chromatin.
Nature Commun 12:146 doi:10.1038/s41467-020-20161-9 (2021) PubMed
2020
- Hori T, Cao J, Nishimura K, Ariyoshi M, Arimura Y, Kurumizaka H, Fukagawa T
Essentiality of CENP-A Depends on Its Binding Mode to HJURP.
Cell Rep 33(7):108388. doi: 10.1016/j.celrep.2020.108388. (2020) PubMed - Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T
A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.
Nucl Acids Res 48, e108. doi: 10.1093/nar/gkaa748. (2020) PubMed - Martins NMC, Cisneros-Soberanis F, Pesenti E, Kochanova NY, Shang WH, Hori T, Nagase T, Kimura H, Larionov V, Masumoto H, Fukagawa T, Earnshaw WC
H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.
J Cell Sci 134, jcs242610. doi: 10.1242/jcs.242610. (2020) PubMed - Saito M, Kagawa N, Okumura K, Munakata H, Isogai E, Fukagawa T, Wakabayashi Y
CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model.
Cancer Sci 111, 2850-2860. (2020) PubMed - Hara M, Fukagawa T
Dynamics of kinetochore structure and its regulations during mitotic progression.
Cell Mol Life Sci 77, 2981-2995. (2020) PubMed - Hori T, Fukagawa T
Artificial generation of centromeres and kinetochores to understand their structure and function.
Exp Cell Res 389,111898. (2020) PubMed - Takizawa Y, Ho CH, Tachiwana H, Matsunami H, Kobayashi W, Suzuki M, Arimura Y, Hori T, Fukagawa T, Ohi MD, Wolf M, Kurumizaka H
Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome.
Structure 28, 44-53. (2020) PubMed
2019
- Watanabe R, Hara M, Okumura EI, Hervé S, Fachinetti D, Ariyoshi M, Fukagawa T
CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization.
J Cell Biol 218, 4042-4062. (2019) PubMed - Hara M, Fukagawa T
Centromere maintenance during DNA replication.
Nature Cell Biol 21, 669-671. (2019) PubMed - Hara M, Fukagawa T
Where is the right path heading from the centromere to spindle microtubules?
Cell Cycle 18, 1199-1211. (2019) PubMed - Eykelenboom JK, Gierliński M, Yue Z, Hegarat N, Pollard H, Fukagawa T, Hochegger H, Tanaka TU
Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis.
J Cell Biol 218, 1531-1552. (2019) PubMed - Arimura Y, Tachiwana H, Takagi H, Hori T, Kimura H, Fukagawa T, Kurumizaka H
The CENP-A centromere targeting domain facilitates H4K20 monomethylation in the nucleosome by structural polymorphism.
Nature Communications 10, 576 doi: 10.1038/s41467-019-08314-x. (2019) PubMed - Nishimura K, Komiya M, Hori T, Itoh T, Fukagawa T
3D genomic architecture reveals that neocentromeres associate with heterochromatin regions.
J Cell Biol 218, 134-149. (2019) PubMed
2018
- Hara M, Ariyoshi M, Okumura EI, Hori T, Fukagawa T
Multiple phosphorylations control recruitment of the KMN network onto kinetochores.
Nature Cell Biol 20, 1378-1388. (2018) PubMed - Hara M, Fukagawa T
Kinetochore assembly and disassembly during mitotic entry and exit.
Curr Opin Cell Biol 52, 73-81. (2018) PubMed - Mills WE, Spence JM, Fukagawa T, Farr CJ
Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.
Int J Mol Sci 19, E534. (2018) PubMed
2017
- Hara M, Fukagawa T
Critical Foundation of the Kinetochore: The Constitutive Centromere-Associated Network (CCAN).
Prog Mol Subcell Biol. 56, 29-57. (2017) PubMed - Okumura K, Kagawa N, Saito M, Yoshizawa Y, Munakata H, Isogai E, Fukagawa T, Wakabayashi Y
CENP-R acts bilaterally as a tumor suppressor and an oncogene in the two-stage skin carcinogenesis model.
Cancer Sci 216, 101-113. (2017) PubMed - Hori T, Shang WH, Hara M, Ariyoshi M, Arimura Y, Fujita R, Kurumizaka H, Fukagawa T
Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.
Dev Cell 42, 181-189. (2017) PubMed - Fukagawa T
Critical histone post-translational modifications for centromere function and propagation.
Cell Cycle 16, 1259-1265. (2017) PubMed - Nishimura K, Fukagawa T
An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9.
Chromosome Res 25, 253-260 (2017) PubMed - Vargiu G, Makarov AA, Allan J, Fukagawa T, Booth DG, Earnshaw WC
Stepwise unfolding supports a subunit model for vertebrate kinetochores.
Proc Natl Acad Sci. USA 114, 3133-3138. (2017) PubMed - Hori T, Kagawa N, Toyoda A, Fujiyama A, Misu S, Monma N, Makino F, Ikeo K, Fukagawa T
Constitutive centromere-associated network controls centromere drift in vertebrate cells.
J Cell Biol 216, 101-113. (2017) PubMed
2016
- Shang WH, Hori T, Westhorpe FG, Godek KM, Toyoda A, Misu S, Monma N, Ikeo K, Carroll CW, Takami Y, Fujiyama A, Kimura H, Straight AF, Fukagawa T
Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres.
Nature Communications 7, 13465 doi: 10.1038/ncomms13465. (2016) PubMed - Abe T, Kawasumi R, Arakawa H, Hori T, Shirahige K, Losada A, Fukagawa T, Branzei D
Chromatin determinants of the inner-centromere rely on replication factors with functions that impart cohesion.
Oncotarget doi: 10.18632/oncotarget.11982. (2016) PubMed - Nagpal H, Fukagawa T
Kinetochore assembly and function through the cell cycle.
Chromosoma 125, 645-659. (2016) PubMed - Dyomin AG, Koshel EI, Kiselev AM, Saifitdinova AF, Galkina SA, Fukagawa T, Kostareva AA, Gaginskaya ER
Chicken rRNA Gene Cluster Structure.
PLoS One 11, e0157464. (2016) PubMed - Nishino T, Fukagawa T
Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.
Methods Mol Biol. 1413, 135-146 (2016) PubMed - Kusakabe M, Oku H, Matsuda R, Hori T, Muto A, Igarashi K, Fukagawa T, Harata M
Genetic complementation analysis showed distinct contributions of the N-terminal tail of H2A.Z to epigenetic regulations.
Genes Cells 21, 122-135 (2016) PubMed - Wood L, Booth DG, Vargiu G, Ohta S, deLima Alves F, Samejima K, Fukagawa T, Rappsilber J, Earnshaw WC
Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability..
Open Biol 6(1):150230. doi: 10.1098/rsob.150230. (2016) PubMed - Furuta M, Hori T, Fukagawa T
Chromatin binding of RCC1 during mitosis is important for its nuclear localization in interphase.
Mol Biol Cell 27, 371-383 (2016) PubMed - Satrimafitrah P, Barman HK, Ahmad A, Nishitoh H, Nakayama T, Fukagawa T, Takami Y
RbAp48 is essential for viability of vertebrate cells and plays a role in chromosome stability.
Chromosome Res 24, 161-173 (2016) PubMed
2015
- Samejima I, Spanos C, Alves Fde L, Hori T, Perpelescu M, Zou J, Rappsilber J, Fukagawa T, Earnshaw WC
Whole-proteome genetic analysis of dependencies in assembly of a vertebrate kinetochore.
J. Cell Biol. 211, 1141-1156 (2015) PubMed - Amakawa Y, Sakata Y, Hoki Y, Arata S, Shioda S, Fukagawa T, Sasaki H, Sado T
A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects.
Development 142, 4299-4308 (2015) PubMed - Nagpal H, Hori T, Furukawa A, Sugase K, Osakabe A, Kurumizaka H, Fukagawa T
Dynamic changes in the CCAN organization through CENP-C during cell-cycle progression.
Mol Biol Cell 26, 3768-3776 (2015) PubMed - Perpelescu M, Hori T, Toyoda A, Misu S, Monma N, Ikeo K, Obuse C, Fujiyama A, Fukagawa T
HJURP is involved in the expansion of centromeric chromatin.
Mol Biol Cell 26, 2742-2754 (2015) PubMed - Fukagawa T
Cell Division: A New Role for the Kinetochore in Central Spindle Assembly.
Curr Biol 25, R554-R557 (2015) PubMed - Ohta S, Wood L, Toramoto I, Yagyu K, Fukagawa T, Earnshaw WC
CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.
Mol Biol Cell 26, 1225-1237 (2015) PubMed
2014
- Arimura Y, Shirayama K, Horikoshi N, Fujita R, Taguchi H, Kagawa W, Fukagawa T, Almouzni G, Kurumizaka H
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3.
Sci Rep 4, 7115 (2014) PubMed - Fukagawa T, Earnshaw WC
Neocentromeres.
Curr Biol 24, R946-R947 (2014) PubMed - Fukagawa T, Earnshaw WC
The centromere: chromatin foundation for the kinetochore machinery.
Dev Cell 30, 496-508 (2014) PubMed - Hori T, Shang WH, Toyoda A, Misu S, Monma N, Ikeo K, Molina O, Vargiu G, Fujiyama A, Kimura H, Earnshaw WC, Fukagawa T
Histone H4 Lys 20 Monomethylation of the CENP-A Nucleosome Is Essential for Kinetochore Assembly.
Dev Cell 29, 740-749 (2014) PubMed - Ishiguro K, Kim J, Shibuya H, Hernández-Hernández A, Suzuki A, Fukagawa T, Shioi G, Kiyonari H, Li XC, Schimenti J, Höög C, Watanabe Y
Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes.
Genes Dev 28, 594-607 (2014) PubMed - Nishibuchi I, Suzuki H, Kinomura A, Sun J, Liu NA, Horikoshi Y, Shima H, Kusakabe M, Harata M, Fukagawa T, Ikura T, Ishida T, Nagata Y, Tashiro S
Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2.
Int J Radiat Oncol Biol Phys. 89, 736-744 (2014) PubMed - Fox D 3rd, Yan Z, Ling C, Zhao Y, Lee DY, Fukagawa T, Yang W, Wang W
The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.
Cell Res 24, 560-575 (2014) PubMed - Kagawa N, Hori T, Hoki Y, Hosoya O, Tsutsui K, Saga Y, Sado T, Fukagawa T
The CENP-O complex requirement varies among different cell types.
Chromosome Res 22, 293-303 (2014) PubMed - Takeuchi K, Nishino T, Mayanagi K, Horikoshi N, Osakabe A, Tachiwana H, Hori T, Kurumizaka H, Fukagawa T
The centromeric nucleosome-like CENP-T-W-S-X complex induces positive supercoils into DNA.
Nucl Acids Res 42, 1644-1655 (2014) PubMed
2013
- Fukagawa T
Speciation mediated by centromeres.
Dev Cell 21, 367-368 (2013) PubMed - Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KH, Copenhaver GP, Deluca JG, Desai A, Diekmann S, Erhardt S, Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM, Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LE, Karpen G, Kops GJ, Lampson MA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RL, Masumoto H, McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O'Neill RJ, Salmon ED, Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE, Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L, Yanagida M, Yen TJ, Yoda K, Cleveland DW
Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant.
Chromosome Res 21, 101-106 (2013) PubMed - Kikuchi K, Narita T, Pham VT, Iijima J, Hirota K, Keka IS, Mohiuddin, Okawa K, Hori T, Fukagawa T, Essers J, Kanaar R, Whitby MC, Sugasawa K, Taniguchi Y, Kitagawa K, Takeda S
Structure-specific endonucleases xpf and mus81 play overlapping but essential roles in DNA repair by homologous recombination.
Cancer Res73, 4362-4371 (2013) PubMed - Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, Fukagawa T
Chromosome engineering allows the efficient isolation of vertebrate neocentromeres.
Dev Cell 24, 635-648 (2013) PubMed - Osakabe A, Tachiwana H, Takaku M, Hori T, Obuse C, Kimura H, Fukagawa T, Kurumizaka H
Vertebrate Spt2 is a novel nucleolar histone chaperone that assists in ribosomal DNA transcription.
J Cell Sci 1323-1332 (2013) PubMed - Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T
CENP-T provides a structural platform for outer kinetochore assembly.
EMBO J 32, 424-436 (2013) PubMed - Hori T, Shang WH, Takeuchi K, Fukagawa T
The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly.
J Cell Biol 200, 45-60 (2013) PubMed
2012
- Krasikova A, Fukagawa T, Zlotina A
High-resolution mapping and transcriptional activity analysis of chicken centromere sequences on giant lampbrush chromosomes.
Chromosome Res 20, 995-1008 (2012) PubMed - Nishimura K, Ishiai M, Horikawa K, Fukagawa, T, Takata M, Takisawa H, Kanemaki MT
Mcm8 and Mcm9 Form a Complex that Functions in Homologous Recombination Repair Induced by DNA Interstrand Crosslinks.
Mol Cell 47, 511-522. (2012) PubMed - Hori, T, Fukagawa T
Establishment of the vertebrate kinetochores.
Chromosome Res 20, 547-561. (2012) PubMed - Fukagawa T
Formation of a centromeric-specific chromatin structure.
Epigenetics 7, 672-675 (2012) PubMed - Maruyama EO, Hori T, Tanabe H, Kitamura H, Matsuda R, Tone S, Hozak P, Habermann FA, von Hase J, Cremer C, Fukagawa T, Harata M
The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.
J Cell Sci 125, 3739-3743 (2012) PubMed - Takeuchi K, Fukagawa T
Molecular architecture of vertebrate kinetochores.
Exp Cell Res 318, 1367-1374 (2012) PubMed - Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T
CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold.
Cell 148, 487-501 (2012) PubMed
2011
- Perpelescu M, Fukagawa T
The ABCs of CENPs.
Chromosoma 120, 425-446 (2011) PubMed - Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM
Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes.
Cell 145, 410-422 (2011) PubMed - Suzuki A, Hori T, Nishino T, Usukura J, Miyagi A, Morikawa K, Fukagawa T
Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins.
J Cell Biol 193, 125-140 (2011) PubMed - Suzuki A, Fukagawa T
Cell Biological Analysis of DT40 Knockout Cell Lines for Cell-Cycle Genes.
Curr Protoc Cell Biol 50, 8.7.1-8.7.17 (2011) PubMed
2010
- Schmidt JC, Kiyomitsu T, Hori T, Backer CB, Fukagawa T, Cheeseman IM
Aurora B kinase controls the targeting of the Astrin/SKAP complex to bi-oriented kinetochores.
J Cell Biol 191, 269-280 (2010) PubMed - Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
The protein composition of mitotic chromosomes determined using multi-classifier combinatorial proteomics.
Cell 142, 810-821 (2010) PubMed - Shang WH, Hori T, Toyoda A, Kato J, Popendorf K, Sakakibara Y, Fujiyama A, Fukagawa T
Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences.
Genome Res 20, 1219-1228 (2010) PubMed - Ribeiro SA, Vagnarelli P, Dong Y, Hori T, McEwen BF, Fukagawa T, Flors C, Earnshaw WC
A super-resolution map of the vertebrate kinetochore.
Proc Natl Acad Sci USA 107, 10484-10489 (2010) PubMed - Johnston K, Joglekar A, Hori T, Suzuki A, Fukagawa T, Salmon ED
Vertebrate Kinetochore Protein Architecture: Protein Copy Number.
J Cell Biol 189, 937-943. (2010) PubMed - Cheng Y, Geng H, Cheng SH, Liang P, Bai Y, Li J, Srivastava G, Ng MH, Fukagawa T, Wu X, Chan AT, Tao Q
The KRAB zinc finger protein ZNF382 is a general, proapoptotic tumor suppressor repressing multiple oncogenes and frequently silenced in multiple carcinomas.
Cancer Res 70, 6516-6526 (2010) PubMed - Welburn JP, Vleugel M, Liu D, Yates JR III, Lampson MA, Fukagawa T, Cheeseman IM
Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface.
Mol Cell 38, 383-392 (2010) PubMed - Matsuda R, Hori T, Kitamura H, Takeuchi K, Fukagawa T, Harata M
Identification and characterization of two isoforms of the vertebrate H2A.Z histone variant.
Nucl Acids Res 38, 4263-4273 (2010) PubMed - Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA.
Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase.
J Cell Biol 188, 809-820 (2010) PubMed
2009
- Xu Z, Ogawa H, Vagnarelli P, Bergmann JH, Hudson DF, Ruchaud S, Fukagawa T, Earnshaw WC, Samejima K
INCENP -aurora B interactions modulate kinase activity and chromosomal passenger complex localization.
J Cell Biol 187, 637-653 (2009) PubMed - Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M
An auxin-based degron system for the rapid deletion of proteins in nonplant cells.
Nature Meth 6, 917-922 (2009) PubMed - Okada M, Okawa K, Isobe T, Fukagawa T
CENP-H-containing complex facitates centromere deposition of CENP-A in cooperation with FACT and CHD1.
Mol Biol Cell 20, 3986-3995 (2009) PubMed - Amano M, Suzuki A, Hori T, Backer C, Okawa K, Cheeseman IM, Fukagawa T
The CENP-S complex is essential for the stable assembly of outer kinetochore structure.
J Cell Biol 186, 173-182 (2009) PubMed - Iwamoto M, Mori C, Kojidani T, Bunai F, Hori T, Fukagawa T, Hiraoka Y, Haraguchi T
Two Distinct Repeat Sequences of Nup98 Nucleoporins Characterize Dual Nuclei in the Binucleated Ciliate Tetrahymena.
Curr Biol 19, 843-847 (2009) PubMed - Kawashima T, Bao YC, Minoshima Y, Nomura Y, Hatori T, Hori T, Fukagawa T, Fukada T, Takahashi N, Nosaka T, Inoue M, Sato T, Kukimoto-Niino M, Shirouzu M, Yokoyama S, Kitamura T
A Rac GTPase activating protein MgcRacGAP is an NLS-containing nuclear chaperone in the activation of STAT transcription factors.
Mol Cell Biol 29, 1796-1813 (2009) PubMed - Kong X, Ball AR Jr, Sonoda E, Feng J, Takeda S, Fukagawa T, Yen TJ, Yokomori K
Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assemble in vertebrate cells.
Mol Biol Cell 20, 1289-1301 (2009) PubMed - Akagi T, Fukagawa T, Kage Y, To H, Matsunaga N, Koyanagi S, Uchida A, Fujii A, Iba H, Ikemura T, Aramaki H, Higuchi S, Ohdo S
Role of glucocorticoid receptor in the regulation of cellular sensitivity to irinotecan hydrochloride.
J Pharmcol Sci 109, 265-274 (2009) PubMed
2008
- Hori T, Amano M, Suzuki A, Backer CB, Welburn JP, Dong Y, McEwen BF, Shang WH, Suzuki E, Okawa K, Cheeseman IM, Fukagawa T
CCAN makes multiple contacts with centromeric DNA and provides distinct pathways to the outer kinetochore
Cell 135, 1039-1052 (2008) PubMed - Hori T, Okada M, Maenaka K, Fukagawa T
CENP-O-class proteins form a stable complex and are required for proper kinetochore function.
Mol Biol Cell 19, 843-854 (2008) PubMed - Cheeseman IM, Hori T, Fukagawa T, Desai A
KNL1 and CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates.
Mol Biol Cell 19, 587-594 (2008) PubMed - Fukagawa T
The kinetochore and spindle checkpoint in vertebrate cells.
Front Biosci 13, 2705-2713 (2008) PubMed
2007
- Kwon MS, Hori T, Okada M, Fukagawa T
CENP-C is involved in chromosome segregation, mitotic checkpoint function and kinetochore assembly.
Mol Biol Cell 18, 2155-2168 (2007) PubMed - Zuccolo M, Alves A, Galy V, Bolhy S, Formstecher E, Racine V, Sibarita JB, Fukagawa T, Shiekhattar R, Yen T, Doye V
The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions.
EMBO J 26, 1853-1864 (2007) PubMed - Takami Y, Ono T, Fukagawa T, Shibahara K, Nakayama T
Essential Role of CAF-1-mediated Rapid Nucleosome Assembly for DNA Replication and Cell Division in Vertebrate Cells.
Mol Biol Cell 18, 129-141 (2007) PubMed
2006
- Kimura H, Takizawa N, Allemand E, Hori T, Iborra FJ, Nozaki N, Muraki M, Hagiwara M, Krainer AR, Fukagawa T, Okawa K
A novel histone-exchange factor, protein phosphatase 2Cg, mediates the exchange and dephosphorylation of H2A/H2B.
J Cell Biol 175, 389-400 (2006) PubMed - Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR III, Desai A, Fukagawa T
The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.
Nature Cell Biol 8, 446-457 (2006) PubMed - Kline SL, Cheeseman IM, Hori T, Fukagawa T, Desai A
The Human Mis12 Complex is Required for Kinetochore Assembly and Proper Chromosome Segregation.
J Cell Biol 173, 9-17 (2006) PubMed - Sanematsu F, Takami Y, Barman HK, Fukagawa T, Ono T, Shibahara K, Nakayama T
Asf1 is required for viability and chromatin assembly during DNA replication in vertebrate cells.
J. Biol. Chem. 281, 13817-13827 (2006) PubMed
2005
- Yamauchi A, Ichimiya T, Inoue K, Taguchi Y, Matsunaga N, Koyanagi S, Fukagawa T, Aramaki H, Higuchi S, Ohdo S
Cell-cycle-dependent pharmacology of methotrexate in HL-60.
J Pharmacol Sci 99, 335-341 (2005) PubMed - Minoshima Y, Hori T, Okada M, Kimura H, Haraguchi T, Hiraoka Y, Bao YC, Kawashima T, Kitamura T, Fukagawa T
The constitutive centromere component CENP-50 is required for recovery from spindle damage.
Mol Cell Biol 25, 10315-10328 (2005) PubMed - Régnier V, Vagnarelli P, Fukagawa T, Zerjal T, Burns E, Trouche D, Earnshaw W, Brown W
CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.
Mol Cell Biol 25, 3967-3981 (2005) PubMed - Mikami Y, Hori T, Kimura H, Fukagawa T
The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis.
Mol Cell Biol 25, 1958-1970 (2005) PubMed - Motohashi T, Shimojima T, Fukagawa T, Maenaka K, Park EY
Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system.
Biochem Biophys Res Commun 326, 564-569 (2005) PubMed
2004
- Fukagawa T
Centromere DNA, proteins and kinetochore assembly in vertebrate cells.
Chromosome Res 12, 557-567 (2004) PubMed - Fukagawa T, Nogami M, Yoshikawa M, Ikeno M, Okazaki T, Takami Y, Nakayama T, Oshimura M
Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
Nature Cell Biol 6, 784-791 (2004) PubMed - Fukagawa T
Assembly of kinetochores in vertebrate cells..
Exp Cell Res 296, 21-27 (2004) PubMed
2003
- Régnier V, Novelli J, Fukagawa T, Vagnarelli P, Brown W
Characterization of chicken CENP-A and comparative sequence analysis of vertebrate centromere-specific histone H3-like proteins.
Gene 316, 39-46 (2003) PubMed - Hori T, Haraguchi T, Hiraoka Y, Kimura H, Fukagawa T
Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells.
J Cell Sci 116, 3347-3362 (2003) PubMed - Hayashi T, Seki M, Maeda D, Wang W, Kawabe Y, Seki T, Saitoh H, Fukagawa T, Yagi H, Enomoto T
Ubc9 is essential for viability of higher eukaryotic cells.
Exp Cell Res 280, 212-221 (2003) PubMed
2002
- Spence JM, Critcher R, Ebersole TA, Valdivia MM, Earnshaw WC, Fukagawa T, Farr CJ
Co-localization of centromere activity, proteins and topoisomerase II within a subdomain of the major human X alpha-satellite array.
EMBO J 21, 5269-5280 (2002) PubMed - Ohno M, Fukagawa T, Lee JS, Ikemura T
Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and antitriplex antibodies.
Chromosoma 111, 201-213 (2002) PubMed - Nishihashi A, Haraguchi T, Hiraoka Y, Ikemura T, Regnier V, Dodson H, Earnshaw WC, Fukagawa T
CENP-I is essential for centromere function in vertebrate cells.
Dev Cell 2, 463-476 (2002) PubMed
2001
- Sonoda E, Matsusaka T, Morrison C, Vagnarelli P, Hoshi O, Ushiki T, Nojima K, Fukagawa T, Waizenegger IC, Peters JM, Earnshaw WC, Takeda S
Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells.
Dev Cell 1, 759-770 (2001) PubMed - Fukagawa T, Regnier V, Ikemura T
Creation and characterization of temperature-sensitive CENP-C mutants in vertebrate cells..
Nucl Acids Res 29, 3796-3803 (2001) PubMed - Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi T, Hiraoka Y, Sugata N, Todokoro K, Brown W, Ikemura T
CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells.
EMBO J 20, 4603-4617 (2001) PubMed - Okamura A, Pendon C, Valdivia MM, Ikemura T, Fukagawa T
Gene structure, chromosomal localization and immunolocalization of chicken centromere proteins CENP-C and ZW10.
Gene 262, 283-290 (2001) PubMed
1999
- Fukagawa T, Pendon C, Morris J, Brown W
CENP-C is necessary but not sufficient to induce formation of a functional centromere.
EMBO J 18, 4196-4209 (1999) PubMed - Fukagawa T, Hayward N, Yang J, Azzalin C, Griffin D, Stewart AF, Brown W
The chicken HPRT gene: a counter selectable marker for the DT40 cell line.
Nucl Acids Res 27, 1966-1969 (1999) PubMed
1997
- Fukagawa T, Brown WR
Efficient conditional mutation of the vertebrate CENP-C gene.
Hum Mol Genet 6, 2301-2308 (1997) PubMed - Tenzen T, Yamagata T, Fukagawa T, Sugaya K, Ando A, Inoko H, Gojobori T, Fujiyama A, Okumura K, Ikemura T
Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex.
Mol Cell Biol 17, 4043-4050 (1997) PubMed - Sugaya K, Sasanuma S, Nohata J, Kimura T, Fukagawa T, Nakamura Y, Ando A, Inoko H, Ikemura T, Mita K.
Gene organization of human NOTCH4 and (CTG)n polymorphism in this human counterpart gene of mouse proto-oncogene Int3.
Gene 189, 235-244 (1997) PubMed