| Sunday, March 11 |
| 12:30 |
Registration |
| 13:20 |
Welcome &Announcement
|
Akihiko Ishijima (Osaka Univ.) |
| Export & Assembly |
|
| Export & Assembly 1 |
Discussion Leader: Naoya Terahara (Osaka Univ.) |
| 13:30-13:45 |
Satoshi Shibata
|
OIST |
Metal ion binding of the Methanococcus archaellin required for filament integrity |
| 13:45-14:00 |
Akinobu Kajikawa
|
Tokyo Univ. Agri.
|
Characterization of flagellins isolated from Lactobacillus agilis |
| 14:00-14:15 |
Yuna Inoue
|
Nagoya Univ.
|
Initiation of the polar flagellar assembly facilitated by FlhF, the positive regulator for flagellar number in Vibrio alginolyticus |
| 14:15-14:30 |
Yoshino Imura
|
Nagoya Univ.
|
Biochemical analysis of FlhG, ATPase Involved in regulating the number of flagella of Vibrio alginolyticus |
| 14:30-14:40 |
Coffee break (10min) |
| |
Discussion Leader: Takayuki Kato (Osaka Univ.) |
| 14:40-14:55 |
Tomohiro Kimura
|
Univ. Tokyo
|
The zoospores of Actinoplanes missouriensis adhere to a solid surface by the type 4 pili |
| 14:55-15:10 |
Akiko Takaya
|
Chiba Univ.
|
The biological function of SsaH as a chaperone in SPI2-T3SS |
| 15:10-15:25 |
Keiya Takata
|
Osaka Univ.
|
Detection of the flagellar type III protein export by fluorescence |
| 15:25-15:50 |
Chien-Jung Lo* |
National Central University |
Toward a more complete understanding of the bacterial flagellar growth |
| 15:50-16:00 |
Coffee break (10min) |
| |
Discussion Leader:Tohru Minamino (Osaka Univ.) |
| 16:00-16:15 |
Dara Niketic |
University of Utah |
The flagellar secretrion signal - a new approach |
| 16:15-17:00 |
Kelly Hughes** |
University of Utah |
The latest from the Hughes lab |
| 17:00-17:30 |
Coffee break and Check-in (30min) |
| 17:30-19:00 |
Akihiko Isijima
Hajime Fukuoka |
Osaka Univ. |
Training course (90min) Optional |
| 19:15-21:15 |
Dinner |
| 21:15- |
Discussion |
|
| Monday, March 12 |
| |
Breakfast |
| Rotor & Stator |
| |
Discussion Leader: Shuichi Nakamura (Tohoku Univ.) |
| 9:00-9:25 |
Tomoko Miyata*
|
Osaka Univ.
|
Structure analysis of the flagellar basal body by electron cryomicroscopy. |
| 9:25-9:40 |
Keiichi Hirano
|
Nagoya Univ.
|
Eluciation of the ring formation mechanism of MS ring which is the center of bacterial flagellar motor formation |
| 9:40-9:55 |
Norihiro Takekawa
|
Osaka Univ.
|
Ring structure of FliL, a flagellar stator associated protein |
| 9:55-10:10 |
Florencia Mengucci
|
Nagoya Univ.
/Universidad Nacional de La Plata
|
FliL roles on the two sets of Bradyrhizobium diazoefficiens flagellar systems |
| 10:10-10:25 |
Coffee break (15min) |
| |
Discussion Leader: Hiroyuki Terashima (Nagoya Univ.) |
10:25-10:40 |
Syun Naganawa
|
Toyo Univ.
|
Elucidation of the ion selectivity mechanism of the Na+, K+-driven flagellar stator from Alkaliphilic Bacillus trypoxylicola |
| 10:40-10:55 |
Taira Mino
|
Nagoya Univ.
|
Detection of structural of marine Vibrio bacterium flagellar stator PomA cytoplasmic loop |
| 10:55-11:10 |
Ai Shinobu
|
Tokyo Tech.
|
Role of Threonine residues in ion permeation for the Na+ driven flagellar motor PomA/PomB: insights from MD simulations |
| 11:10-11:25 |
Koki Nishiuchi
|
Osaka Univ.
|
Structure of a periplasmic fragment of MotS from Bacillus alcalophilus |
| 11:25-11:40 |
Tatsuya Morishita
|
NICT |
Directed evolution of motAB using in vivo mutagenesis |
| 11:40-11:55 |
Group Photo |
| 11:55-13:00 |
Lunch |
| Chemotaxis |
| |
Discussion Leader: Norihiro Talekawa (Osaka Univ.) |
| 13:00-13:15 |
So-ichiro Nishiyama
|
Hosei Univ.
|
Temperature-dependent control of a taurine chemoreceptor Mlp37 of Vibrio cholerae |
| 13:15-13:30 |
Yohei Takahashi
|
Osaka Univ.
|
Distinct mechanisms of ligand recognition between Mlp24 and Mlp37, chemoreceptor proteins of Vibrio cholera |
| 13:30-13:45 |
Yuki Miura
|
Hosei Univ.
|
Rotation control of the single polar fagellum of Vibrio cholerae |
| 13:45-14:00 |
Mariko Momma
|
Hosei Univ.
|
Transducers and soluble receptors for chemotaxis of Vibrio alginolyticus |
| 14:00-14:15 |
Coffee break (15min) |
| |
Discussion Leader: Yusuke Morimoto (Kyutech) |
| 14:15-14:30 |
Shin-Ichi Aizawa
|
PHU
|
What is the Kapsel? |
| 14:30-14:45 |
Hiroto Tanaka
|
NICT |
Relationship between chemotactic response and size of E. coli. |
14:45-15:00 |
Hiroaki Hata
|
Toko Univ.
|
Pressure-Induced CheY-FliM Dissociation Simulated by Parallel Cascade Selection Molecular Dynamics |
| 15:00-15:25 |
Hajime Fukuoka
|
Osaka Univ.
|
Single cell measurement for the chemotaxis proteins and cellular behavior |
| 15:25-15:40 |
|
Nikon.
|
Technicologies |
| 15:40-15:55 |
Coffee break (15min) |
| |
Discussion Leader: Seiji Kojima (Nagoya Univ.) |
| 15:55-16:10 |
Jerko Rosko |
University of Edinburgh |
Swimming in complex environments |
| 16:10-16:55 |
John (Sandy) Parkinson
** |
University of Utah |
Transmembrane Signaling Mechanisms of E. coli Chemoreceptors |
| 16:55-17:10 |
Coffee break (15min) |
| 17:10-19:00 |
Akihiko Ishijima
Hajime Fukuoka |
Osaka Univ. |
Training course (90min) Optional |
| 19:15-21:15 |
Dinner |
| 21:15- |
Discussion |
|
|
| Tuesday, March 13 |
| |
Breakfast |
| Filament |
|
| |
Discussion Leader: Satoshi Sibata (OIST) |
| 9:00-9:25 |
Masayoshi Nishiyama*
|
Univ. Tokyo.
|
Visualizing flagellar motions of magnetotactic bacterium MO-1 |
| 9:25-9:40 |
Peter Horvath |
Osaka Univ.
|
High resolution structural analysis of the flagellar hook of Salmonella enterica serovar Typhimurium |
| 9:40-9:55 |
Kodai Okada
|
Osaka Univ.
|
Structure of FimA, a major component protein of fimbriae of Porphyromonas gingivalis |
| 9:55-10:10 |
Fumiaki Makino
|
Osaka Univ.
|
The cap structure of the bacterial flagellar filament |
| 10:10-10:25 |
Saijo Yumiko
|
Osaka Univ.
|
Structure of the flagellar distal rod constructed by full-length FlgG |
| 10:25-10:35 |
Coffee break (10min) |
| Flagellar Motor |
|
| |
Discussion Leader: Takashi Sagawa (NICT) |
| 10:35-10:50 |
Tomofumi Sakai
|
Osaka Univ.
|
Functional analysis of a FliF-G deletion-fusion mutant flagellar motor |
| 10:50-11:05 |
Taishi Kasai
|
Hosei Univ.
|
The effect of MotB(D32E) on the stall torque of Escherichia coli flagellar motor |
| 11:05-11:20 |
Yuya Suzuki
|
Tohoku Univ.
|
Effect of temperature on assembly of the flagellar motor stator |
| 11:20-11:30 |
Coffee break (10min) |
| |
Discussion Leader: Masayoshi Nishiyama (Kyoto Univ.) |
| 11:30-11:45 |
Tsubasa Ishida
|
Hosei Univ.
|
Measurement of flagellar motor rotation at low load |
| 11:45-12:00 |
Yuta Hanaizumi
|
Tohoku Univ.
|
Rotation assay of the flagellar motor under near zero load |
| 12:00-12:15 |
Seiji Iwata
|
Gakushuin Univ.
|
3D torque measurements of the archaeal motor |
| 12:15-12:30 |
Naoya Terahara
|
Osaka Univ.
|
Step analysis of Bacillus flagellar motor |
| 12:30 |
Conference Closing |
Akihiko Ishijima (Osaka Univ.) |
