Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
Journal | Sci Rep 7, 42398 (2017) |
---|---|
Title | Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging |
Abstract
We report development of the first genetically encoded bioluminescent indicator for membrane voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require external light illumination. This allows bidirectional optogenetic control of cellular activity triggered by Channelrhodopsin2 and Halorhodopsin during voltage imaging. The other advantage of LOTUS-V is the robustness of a signal-to-background ratio (SBR) wherever it expressed, even in the specimens where autofluorescence from environment severely interferes fluorescence imaging. Through imaging of moving cardiomyocyte aggregates, we demonstrated the advantages of LOTUS-V in long-term imaging are attributable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with the ratiometric aspect of LOTUS-V design. Collectively LOTUS-V extends the scope of excitable cell control and simultaneous voltage phenotyping, which should enable applications in bioscience, medicine and pharmacology previously not possible.
Authors | Inagaki S (1), Tsutsui H (2, 3), Suzuki K (4), Agetsuma M (5), Arai Y (5), Jinno Y (3), Bai G (5), Daniels MJ (6), Okamura Y (1, 3), Matsuda T (5), Nagai T (1, 4, 5)
|
---|---|
PubMed | 28205521 |