Movie 1 - Movie of iFRAP of Hec1-GFP during G2 phase.

 

Movie 2 - Movie of iFRAP of Hec1-GFP during mitosis.

 

Movie 3 - Movie of iFRAP of CENP-H-GFP during interphase.

 

Movie 4 - Movie of iFRAP of CENP-H-GFP during mitosis.

Supplement 5 - (A) Two-hybrid analysis of interactions between human CENP-Hs (left) and chicken CENP-Hs (right) to investigate multimerization of CENP-H. As a positive control, interaction between T-antigen (AD/T antigen fusion) and p53 (BD/p53 fusion) is shown.
(B) Co-immunoprecipitation analysis to investigate the interaction of CENP-H and CENP-H-Flag to investigate multimerization of CENP-H. CHF3 cells, which express CENP-H and CENP-H-Flag in the absence of tet, were prepared, and mitotic extracts of CHF3/-tet cells were immunoprecipitated with anti-Flag antibody. Immunoprecipitates were separated by SDS-PAGE and analyzed by Western blotting with anti-CENP-H. We detected only the CENP-H-Flag band.