Integrated Biology Laboratories
Laboratory of Intracellular Membrane Dynamics
Autophagy, Membrane traffic, Lysosome, Disease, Aging
Why do cells eat themselves?
Eukaryotic cells are equipped with the intracellular traffic network by witch a variety of macromolecules intercommunicate among organelles. The traffic is executed by dynamic membrane movement. so-called membrane traffic. We focus on autophagy, the membrane traffic delivering cargos from the cytoplasm to lysosomes for degradation, by using advanced methodologies of molecular cell biology, imaging, etc. Autophagy field is one of the hottest fields in life science. Prof Yoshimori in the lab has been leading the field together with Prof Ohsumi who received the 2016 Nobel Prize. He expanded Ohsumi’s breakthrough using yeast to mammalian, and has been leading autophagy field by elucidating molecular machinery and anti-diseases function of mammalian autophagy.
Fig1. The membrane dynamics of autophagy
Fig2. Autophagosome is engulfing the invading pathogenic bacteria (arrow). We reported for the first time that autophagy protects cells against pathogenic bacteria.
|Tamotsu Yoshimori (Professor)||tamyoshi[at]fbs.osaka-u.ac.jp|
|Maho Hamasaki (Associate Professor)||hamasaki[at]fbs.osaka-u.ac.jp|
|Shuhei Nakamura (Associate Professor, Institute for Advanced Co-Creation Studies)||shuhei.nakamura[at]fbs.osaka-u.ac.jp|
|Keisuke Tabata (Assistant Professor)||keisuke.tabata[at]fbs.osaka-u.ac.jp|
|Tatsuya Kaminishi (Assistant Professor, Graduate School of Medicine)||tkaminishi[at]fbs.osaka-u.ac.jp|
|Akiko Kuma (Specially Appointed Associate Professor, Graduate School of Medicine)||kuma[at]fbs.osaka-u.ac.jp|
|Hitomi Imoto (Specially Appointed Assistant Professor, Graduate School of Medicine)||himoto[at]fbs.osaka-u.ac.jp|
|Satoshi Minami (Specially Appointed Assistant Professor, Graduate School of Medicine)|
|Takayuki Shima (Specially Appointed Researcher, Graduate School of Medicine)||tkshima[at]fbs|
|Eriko Ochi (Secretary, Graduate School of Medicine)|
|Kayo Murata (Secretary, Graduate School of Medicine)|
|Ayaka Tokumura (Technical Staff, Graduate School of Medicine)|
|Akiko Nezu (Technical Staff, Graduate School of Medicine)|
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- What is your hot research topic?
- We focus on the molecular mechanism of Autophagy and its correlation to some diseases and aging.
- What kind of background do your lab members have？
- Most of staffs have some expertise of Cell biology. In 2019 we got a new specialist for Structure Biology in our lab. Grad. Students are from a variety of area like faculty of Science (biology), Agriculture, Medicine, Pharmaceutical Sciences and Engineering.
- Do you collaborate with institutions outside of Osaka Univ.?
- We are in collaboration with lots of research institutes both within and outside of Japan. Specially, we have quite active international collaboration projects through HFSP Grant
(with USA, Germany Australia) and A3 Foresight program (with China and Korea).
- How do you develop your research?
- We will continue our detailed analysis of molecular function in mammalian autophagy. We also will promote the incorporation of our research results into real-life applications. To this end, we recently started a venture named "AutophagyGo".
Publications (Research Articles, Reviews, Books)
Starvation-induced autophagy via calcium-dependent TFEB dephosphorylation is suppressed by Shigyakusan.
PLoS One 15(3):e0230156 2020
Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.
Autophagy 1月16日 2020
Evidence for lysosomal biogenesis proteome defect and impaired autophagy in preeclampsia.
Autophagy 1月15日 2019
Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development
Autophagy 1月15日 2019
Transgenic expression of a ratiometric autophagy probe specifically in neurons enables the interrogation of brain autophagy in vivo
Autophagy 15(3):543-557 2019
Distinct functions of ATG16L1 isoforms in membrane binding and LC3B lipidation in autophagy-related processes
Nat. Cell Biol. 21(3):372-383 2019
Suppression of autophagic activity by Rubicon is a signature of aging
Nat. Commun. 10(1):847 2019
Bidirectional Control of Autophagy by BECN1 BARA Domain Dynamics
Mol. Cell 73(2):339-353 2019
An ATG16L1-dependent pathway promotes plasma membrane repair and limits Listeria monocytogenes cell-to-cell spread
NAT. MICROBIOL 3(12):1472_1485 2018
Induction of selective autophagy in cells replicating hepatitis C virus genome
J. Gen. Virol. 99(12):1643-1657 2018
Transgenic expression of a ratiometric autophagy probe specifically in neurons enables the interrogation of brain autophagy in vivo. Autophagy
Autophagy 15(3):543-557 2018
Trophoblast-Specific Conditional Atg7 Knockout Mice Develop Gestational Hypertension.
Am. J. Pathol. 188(11):2474-2486 2018
A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.
J. Biol. Chem. 293(30):11809-11822 2018
PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.
Embo J. 37(4):e97909 2018
Dysregulation of autophagy in melanocytes contributes to hypopigmented macules in tuberous sclerosis complex.
J. Dermatol. Sci. 89(2):155-164 2018
Autophagy and Longevity.
Mol. Cells 41(1):65-72 2018
Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.
Mol. Cell 67(3)471-483 2017
Molecular definitions of autophagy and related processes
Embo J. 36(13):1811-1836 2017
Autophagy and kidney inflammation
Autophagy 13(6):997-1003 2017
Ubiquitination of exposed glycoproteins by SCFFBXO27 directs damaged lysosomes for autophagy
Proc. Natl. Acad. Sci. U. S. A. 114(32):8574-8579 2017
Small nucleoli are a cellular hallmark of longevity
Nat. Commun. 8:16083 2017
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense
Nat. Immunol. 18(8):899-910 2017
Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement
Sci Rep 7(1):3552 2017
Autophagy Inhibits the Accumulation of Advanced Glycation End Products by Promoting Lysosomal Biogenesis and Function in the Kidney Proximal Tubules
Diabetes 66(5):1359-1372 2017
Endothelial cells are intrinsically defective in xenophagy of Streptococcus pyogenes
PLoS Pathog. 13(7):e1006444 2017
Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis.
Sci Rep 7:44795 2017
Intracellular fate of Ureaplasma parvum entrapped by host cellular autophagy.
MicrobiologyOpen 6(3):e00441 2017
Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Hum. Mol. Genet. 26(1):173-183 2017
New insights into autophagosome-lysosome fusion.
J. Cell Sci. 130(7):1209-1216 2017
A Structurally and Functionally Common Unit in Testes and Ovaries of Medaka
Sex. Dev. 10:159-165 2016
LIN-28 balances longevity and germline stem cell number in Caenorhabditis elegans through let-7/AKT/DAF-16 axis
Aging Cell 16(1):113-124 2016
Regulation of lysosomal phosphoinositide balance by INPP5E is essential for autophagosome-lysosome fusion.
Autophagy 12(12):2500-2501 2016
Rubicon inhibits autophagy and accelerates hepatocyte apoptosis and lipid accumulation in nonalcoholic fatty liver disease.
Hepatology 64(6):1994-2014 2016
Atg9A Trafficking Through the Recycling Endosomes is Required for Autophagosome Formation.
J. Cell Sci. 129(20):3781-3791 2016
Autophagosome-lysosome fusion in neurons requires INPP5E, a protein associated with Joubert syndrome
Embo J. 35(17):1853-67 2016
Ideal Candidate (To Graduate Student)
We are looking for a highly motivated person to work on our research topics as our lab member. Our lab welcomes the person who loves taking care of creatures, hand working and handcraft too. Any kind of background (such as your expertise or major) is available.
Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, Osaka University,
2-2 Yamadaoka, Suita, Osaka 565-0871 Japan.
E-mail: tamyoshi[at]fbs.osaka-u.ac.jp (Prof. Tamotsu Yoshimori)
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