Research

Structural analysis of glycoproteins and glycan-related proteins

The Project Leader's Profile

Yoshiki Yamaguchi

Team Leader, Structural Glycobiology Team, RIKEN

Yoshiki Yamaguchi received his Ph.D. in 1998 at Graduate School of Pharmaceutical Sciences, the University of Tokyo (Profs. Ichio Shimada and Yoji Arata).  He continued his research as a Postdoctoral Research Fellow and then as a Research Associate in the same group.  From 2001 to 2007 he was a Lecturer at Nagoya City University (Prof. Koichi Kato) and then move to his current position at RIKEN, Systems Glycobiology Research Group as a team leader of Structural Glycobiology Team.  His current study focuses on development of NMR techniques for structural analyses of glycoproteins and glycan-related proteins.  He has been holding posts concurrently in Nagoya City University (2007-) and in Tokyo Medical and Dental University (2008-) as a visiting Professor.  He received Young Scientist Awards from GlycoTOKYO in 2006 and from the Japanese Society of Carbohydrate Research in 2007.

Project Leader
  • Yoshiki Yamaguchi (Structural Glycobiology Team, Team Leader)
Research Members
  • Mayumi Kanagawa (Structural Glycobiology Team, Researcher)
  • Tadashi Satoh (Structural Glycobiology Team, Researcher)
  • Shinya Hanashima (Structural Glycobiology Team, Researcher)
  • Akemi Ikeda (Structural Glycobiology Team, Technical Staff)
  • Masaki Kato (Structural Glycobiology Team, Technical Staff)
  • Kana Matsumoto (Structural Glycobiology Team, Technical Staff)

Summary

In order to elucidate the functions of the glycans attached to proteins, it is important to analyze the glycans from the structural point of view. Our team aim to reveal the functions of glycans through the three-dimensional structures of glycoproteins and oligosaccharide-related proteins. We prepare the glycoconjugates samples with the techniques of organic synthesis and cell-engineering, and determine the three-dimensional structures using biophysical methods such as NMR or X-ray crystallography. Furthermore, we construct databases of oligosaccharide structures using bioinformatics-based approaches and decode the 'glycosignals' from the structural information. We particularly focus on disease-related glycoproteins, and develop diagnostic and therapeutic agents.

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Some of Recent Papers

  1. Yamaguchi Y, Wälchli M, Nagano M, Kato K. A 13C-detection NMR approach for large glycoproteins. Carbohydr. Res. 2009 in press
  2. Utsumi M, Yamaguchi Y, Sasakawa H, Yamamoto N, Yanagisawa K, Kato K. Up-and-down topological mode of amyloid β-peptide lying on hydrophilic/hydrophobic interface of ganglioside clusters. Glycoconj. J. 2009 in press
  3. Kato K, Sasakawa H, Kamiya Y, Utsumi M, Nakano M, Takahashi N, Yamaguchi Y. 920 MHz ultra-high field NMR approaches to structural glycobiology. Biochim. Biophys. Acta 1780, 619-625, 2008.
  4. Yamaguchi Y, Hirao T, Sakata E, Kamiya Y, Kurimoto E, Yoshida Y, Suzuki T, Tanaka K, Kato K. Fbs1 protects the malfolded glycoproteins from the attack of peptide:N-glycanase. Biochem. Biophys. Res. Commun. 362, 712-716, 2007.
  5. Matsumiya S, Yamaguchi Y, Saito J, Nagano M, Sasakawa H, Otaki S, Satoh M, Shitara K, Kato K. Structural comparison of fucosylated and nonfucosylated Fc fragments of human immunoglobulin G1. J Mol Biol. 368, 767-779, 2007.