〒565-0871 大阪府吹田市山田丘1−3

大阪大学大学院生命機能研究科ミトコンドリア動態学研究室

©2010 N. & K. Okamoto. All rights reserved. 禁無断転載




Publication list


2018


Mitochondrial stasis reveals p62-mediated ubiquitination in Parkin-independent mitophagy.

Yamada T, Murata D, Adachi Y, Itoh K, Kameoka S, Igarashi A, Dawson TM, Yanagawa T, Okamoto K, Iijima M, *Sesaki H.

Cell Metab., 28(4):588-57 (2018).


The TORC1 signaling pathway regulates respiration-induced mitophagy in yeast.

Liu Y, *Okamoto K.

Biochem. Biophys Res Commun. , doi: 10.1016/j.bbrc.2018.04.114(2018).


The ER membrane insertase Get1/2 is required for efficient mitophagy in yeast.

Onishi M, Nagumo S, Iwashita S, *Okamoto K.

Biochem. Biophys Res Commun., doi: 10.1016/j.bbrc.2018.05.123 (2018).


The Nem1-Spo7 protein phosphatase complex is required for efficient mitophagy in yeast.

Xu X, *Okamoto K.

Biochem. Biophys Res Commun., 496(1):51-57 (2018).


<Reviews, Proceedings and Books>


Phosphatidic acid and cardiolipin coordinate mitochondrial dynamics.

Kameoka S, Adachi Y, Okamoto K, Iijima M, *Sesaki H.

Trends Cell Biol., 28: 67-76. (2018).



分解標識タンパク質が駆動するミトコンドリア特異的オートファジー

*岡本浩二

DOJIN NEWS, No. 165, 7-9., ISSN 0385-1516 (2018).


2017


Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting.

Nagumo S, *Okamoto K.

Methods Mol Biol., doi: 10.1007/7651_2017_11 (2017).


Assays for Mitophagy in Yeast.

Eiyama A, *Okamoto K.

Methods Mol Biol., 1567:337-347. (2017)


<Reviews, Proceedings and Books>


ミトコンドリア分別・除去システムの基本原理

*岡本浩二

The Frontiers in Life Sciencesシリーズ、「オートファジー」, 113-123., ISBN 978-4-525-13481-5 (2017).


2015


Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling

Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, *Okamoto K.

EMBO J. 34: 2703-2719. (2015)


Protein N-terminal acetylation by the NatA complex is critical for selective mitochondrial degradation.

Eiyama A, *Okamoto K.

J. Biol. Chem. 290: 25034-25044.(2015)


<Reviews, Proceedings and Books>


Preface - The special issue on mitophagy.

*Okamoto K.

Biochim. Biophys. Acta 1853: 2755. (2015)


PINK1/Parkin-mediated mitophagy in mammalian cells.

Eiyama A, *Okamoto K.

Curr. Opin. Cell Biol. 33: 95-101.(2015)


2014


Enhancement of ethanol fermentation in Saccharomyces cerevisiae sake yeast by disrupting mitophagy function.

Shiroma S, Jayakody LN, Horie K, Okamoto K, *Kitagaki H.

Appl. Environ. Microbiol.80: 1002-1012.(2014)


<Reviews, Proceedings and Books>


Receptor-mediated mitophagy in yeast and mammalian systems.

Liu L, Sakakibara K, *Chen Q, *Okamoto K.

Cell Res. 24: 787-795.(2014)


Organellophagy: eliminating cellular building blocks via selective autophagy.

*Okamoto K.

J. Cell Biol. 205: 435-445.(2014)


Assays for autophagy II: Mitochondrial autophagy.

*Kanki T, Okamoto K.

Methods Mol. Biol. 1163: 165-173.(2014)


出芽酵母が語る選択的ミトコンドリア分解の基本原理.

*岡本浩二.

医学のあゆみ・AYUMI「マイトファジー―基礎から疾患との関連まで」 250: 483-487.(2014)


2013


Mitochondrial degradation during starvation is selective and temporally distinct from bulk autophagy in yeast.

Eiyama A, Kondo-Okamoto N, *Okamoto K.

FEBS Lett. 587: 1787-1792.(2013)


<Reviews, Proceedings and Books>


No ATG is an island - the connection of autophagy with diverse pathways and functions.

*Komatsu M, Kuma A, Okamoto K.

EMBO Rep. 14: 219-221.(2013)


2012


Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy.

Kondo-Okamoto N, Noda NN, Suzuki SW, Nakatogawa H, Takahashi I, Matsunami M, Hashimoto A, Inagaki F, Ohsumi Y, *Okamoto K .

J. Biol. Chem.287: 10631-10638.(2012)


<Reviews, Proceedings and Books>


Mitochondria and autophagy: critical interplay between the two hemostats.

Kondo-Okamoto N, *Okamoto K.

Biochim. Biophys. Acta 1820: 595-600.(2012)


マイトファジーの分子機構.

*岡本浩二

DOJIN BIOSCIENCE SERIES 04,「オートファジー」. 133-149.(2012)


ミトコンドリア品質管理研究の最前線.

*岡本浩二

細胞 THE CELL 44: 45-48.(2012)


2011


Structure-function analysis of the yeast mitochondrial Rho GTPase, Gem1p: implications for mitochondrial inheritance.

Koshiba, T., Holman, H.A., Kubara, K., Yasukawa, K., Kawabata, S., Okamoto, K., MacFarlane, J., Shaw, J.M.

J. Biol. Chem. 286: 354-362. (2011)


<Reviews, Proceedings and Books>


Mitochondria breathe for autophagy.

Okamoto, K.

EMBO J. 30: 2095-2096.(2011)


Mitochondria autophagy in yeast.

Kanki, T., Klionsky, D.J., Okamoto, K.

Antioxid. Redox Signal.14:1989-2001.(2011)


2010


<Reviews, Proceedings and Books>


酵母のマイトファジー〜観察からの始まり〜.顕微鏡

岡本徳子,岡本浩二,大隅良典

45: 83-86.(2010)


マイトファジー:ミトコンドリアを丸ごと分別・除去する仕組み.細胞工学・特集「その時ミトコンドリアは動いた」

岡本浩二*,岡本徳子

細胞工学・特集「その時ミトコンドリアは動いた」29: 423-428.(2010)


2009


Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy.

Okamoto, K.*, Kondo-Okamoto, N., and Ohsumi, Y.*

Dev. Cell,17: 87–97.(2009)


<Reviews, Proceedings and Books>


A landmark protein essential for mitophagy. Autophagy,

Okamoto, K.*, Kondo-Okamoto, N., and Ohsumi, Y.*

Autophagy, 5: 1203-1205.(2009)


2008


TPR proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis.

Kondo-Okamoto, N., Shaw, J.M., and Okamoto, K.*

EMBO rep., 9: 63-69.(2008)


Multiple pathways influence mitochondrial inheritance in budding yeast.

Frederick, R.L., Okamoto, K., and Shaw, J.M.

Genetics, 178: 825-837.(2008)


2006


The novel F-box protein Mfb1p regulates mitochondrial connectivity and exhibits asymmetric localization in yeast.

Kondo-Okamoto, N., Ohkuni, K., Kitagawa, K., McCaffery, J.M., Shaw, J.M.*, and Okamoto, K.*

Mol. Biol. Cell,17: 3756-3767. (2008)


2005


<Reviews, Proceedings and Books>


Mitochondrial morphology and dynamics in yeast and multicellular eukaryotes.

Okamoto, K.*, and Shaw, J.M.*

Ann. Rev. Genet.,39: 503-536.(2005)


2004


Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway.

Frederick, R.L., McCaffery, J.M., Cunningham K.W., Okamoto, K.*, and Shaw, J.M.*

J. Cell Biol., 167: 87-98. (2004)


Up-to-dating of complete sequenced DNA data of Hansenula wingei yeast mitochondria.

Sekito, T., Okamoto, K., Kitano, H., and Yoshida, K.

Nucleic Acids Symp. Ser., 48: 179-180.(2004)


Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology.

Stojanovski, D., Koutsopoulos, O.S., Okamoto, K., and Ryan, M.T.

J. Cell Sci., 117: 1201-1210.(2004)


2003


Mmm1p spans both the outer and inner mitochondrial membranes and contains distinct domains for targeting and foci formation.

Kondo-Okamoto, N., Shaw, J.M.*, and Okamoto, K.*

J. Biol. Chem., 278: 48997-49005.(2003)


2002


The protein import motor of mitochondria: a targeted molecular ratchet driving unfolding and translocation.

Okamoto, K., Brinker, A., Paschen, S.A., Moarefi, I., Hayer-Hartl, M., Neupert, W., and Brunner, M.

EMBO J., 21: 3659-3671.(2002)


Mitochondrial protein import: molecular basis of the ATP-dependent interaction of mtHsp70 with Tim44.

Moro, F., Okamoto, K., Donzeau, M., Neupert, W., and Brunner, M.

J. Biol. Chem., 277: 6874-6880.(2002)


2001


Replication and preferential inheritance of hypersuppressive petite mitochondrial DNA.

MacAlpine, D.M., Kolesar, J., Okamoto, K., Butow, R.A., and Perlman, P.S.

EMBO J.,20: 1807-1817.(2001)


<Reviews, Proceedings and Books>


Targeting of green fluorescent protein to mitochondria.

Okamoto, K., Perlman, P.S., and Butow, R.A.

Methods Cell Biol., 65: 277-283.(2001)


1998


The sorting of mitochondrial DNA and mitochondrial proteins in zygotes: preferential transmission of mitochondrial DNA to the medial bud.

Okamoto, K., Perlman, P.S., and Butow, R.A.

J. Cell Biol., 142: 613-623.(1998)


Function of the HMG box protein, Abf2p, in mitochondrial DNA segregation, recombination and copy number in Saccharomyces cerevisiae.

Zelenaya-Troitskaya, O., Newman, S.M., Okamoto, K., Perlman, P.S., and Butow, R.A.

Genetics, 148: 1763-1776.(1998)


1996


The secondary structure and phylogenetic relationship deduced from complete nucleotide sequence of mitochondrial small subunit rRNA in yeast Hansenula wing.

Okamoto, K., Sekito, T., and Yoshida, K.

Genes Genet. Syst., 71: 69-74.(1996)


Is the evolution of mitochondrial genes independent from that of the corresponding nuclear genes in fungi? Construction of molecular phylogenetic trees based on mitochondrial small rRNA.

Yoshida, K., Okamoto, K., and Sekito, T.

Nucleic Acids Symp. Ser.,35: 275-276.(1996)


1995


Clone bank of the mitochondrial genome of yeast Hansenula wingei.

Sekito, T., Okamoto, K., Kitano, H., and Yoshida, K.

Yeast, 11: 1317-1321.(1995)


The complete mitochondrial DNA sequence of Hansenula wingei reveals new characteristics of yeast mitochondria.

Sekito, T., Okamoto, K., Kitano, H., and Yoshida, K.

Curr. Genet., 28: 39-53.(1996)


1994


Yeast Hansenula wingei mitochondrial genome's complete DNA sequence demonstrated unique characteristics.

Sekito, T., Okamoto, K., Kitano, H., and Yoshida, K.

Nucleic Acids Symp. Ser., 31: 233-234.(1994)


The mitochondrial genome of yeast Hansenula wingei encodes NADH dehydrogenase subunit genes ND4L and ND5.

Okamoto, K., Sekito, T., and Yoshida, K.

Mol. Gen. Genet., 243: 473-476.(1994)


<Reviews, Proceedings and Books>


酵母ミトコンドリアゲノムの構造とその多様性.

岡本浩二, 関藤孝之, 北野広道, 吉田和夫

蛋白質・核酸・酵素, 39: 1638-1650.(1994)


1993


Nucleotide sequences and secondary structures of four tRNA genes in mitochondrial DNA of Hansenula wingei yeast.

Sekito, T., Okamoto, K., Suzuki, K., and Yoshida, K.

Nucleic Acids Res.,21: 3589.(1993)


1992

Nucleotide sequences of ten mitochondrial tRNA genes in yeast Hansenula wingei.

Okamoto, K., Suzuki, K., and Yoshida, K.

Nucleic Acids Res., 20: 2373.(1992)


1991

Physical mapping and RFLP analysis of mtDNAs from the ascosporogenous yeasts: Saccharomyces exiguus, S. kluyveri and Hansenula wing.
Okamoto, K., Suzuki, K., and Yoshida, K.
Jpn. J. Genet., 66: 709-718.(1991)