おもろい研究!君ならできる、ここでできる|新しい生物学・生命科学を拓く大学院|大阪大学大学院生命機能研究科

English

RELION-3
〜new tools for automated high-resolution cryo-EM structure determination〜
Sjors Scheres (MRC Laboratory of Molecular Biology)

日時

October 27, 2018 (Sat), 13:00-14:00

場所

1F Seminar Room, Nanobiology Building

演者

Sjors Scheres (MRC Laboratory of Molecular Biology)

演題

RELION-3

要旨

I will describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations. Reference-free autopicking with Laplacian-of-Gaussian filtering and execution of jobs from python allows non-interactive processing during acquisition, including 2D-classification, de novo model generation and 3D-classification. Multi-body refinement provides a new tool for describing molecular motions in large, multi-domain complexes. Bayesian polishing provides improved beam-induced motion correction and radiation-damage weighting. Per-particle refinement of CTF parameters and correction of estimated beam tilt provides higher-resolution reconstructions when particles are at different heights in the ice, and/or coma-free alignment has not been optimal. Ewald sphere curvature correction improves resolution for large particles. Using a variety of publicly available data sets, I will illustrate how these developments lead to resolution improvements of 0.2-0.7Å compared to previous versions.

世話人

Keiichi Namba
Tel: 06-6879-4625
E-mail: keiichi[at]fbs.osaka-u.ac.jp