おもろい研究!君ならできる、ここでできる|新しい生物学・生命科学を拓く大学院|大阪大学大学院生命機能研究科

English

生命機能研究科研究交流会(FBSコロキウム)245

講演者 堀 哲也 (准教授/生命機能研究科 染色体生物学研究室(深川研))
演題 高等動物セントロメアのエピジェネティック制御
日時 2020年7月16日(木)12:15〜13:00
場所 Zoomでのオンラインセミナーとなります:参加に必要なサインイン情報等は前日までに、関係者へのメールにてアナウンス致します。
世話人 世話人:堀 哲也(准教授・染色体生物学研究室(深川研))
Name: Tetsuya Hori
Tel:06-6879-4425
E-mail:thori@fbs.osaka-u.ac.jp
言語 英語

演題/要旨

高等動物セントロメアのエピジェネティック制御

The centromere is an essential genome region where the kinetochore is established for faithful chromosome segregation. In most organisms, centromeres are specified by sequence-independent epigenetic mechanisms through the deposition of histone H3-variant CENP-A into chromatin. Therefore, it is critical to know how CENP-A is deposited into centromeric chromatin. We previously showed that the chicken Mis18 complex directly binds to the CENP-A nucleosome and is recognized by the pre-deposition CENP-A-H4-HJURP (CENP-A specific chaperon) complex for new CENP-A incorporation. However, it is still unknown how the pre-deposition CENP-A-H4-HJURP complex correctly transfers the CENP-A into centromeric chromatin and how the CENP-A nucleosome triggers to assemble the kinetochore in the vertebrate cells. Here, we analyzed various CENP-A domains in the chicken DT40 cells, using a gene complementation assay. We found that while both N- and C-terminal tails of CENP-A were dispensable, the alpha-1 helix region near CATD (CENP-A targeting domain) was essential for CENP-A deposition and centromere maintenance in chicken DT40 cells, which is not the case for human CENP-A. Combined biochemical data, we propose that the essentiality of CENP-A depends on its binding mode to HJURP, which is variable during evolution.


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