JEOL YOKOGUSHI Research Alliance Laboratories

The Namba Lab

Graduate School of Frontier Biosciences - Publications

Recent Publications


  • Complementary use of electron cryomicroscopy and X-ray crystallography: structural studies of actin and actomyosin filaments.
    Fujii, T, Namba, K.
    Integrative Structural Biology with Hybrid Methods, Advances in Experimental Medicine and Biology, Springer Nature Singapore Pte Ltd. 
    1105, 25-42 
    (2018)
    PMID: 30617822
  • Mutational analysis of the C-terminal cytoplasmic domain of FlhB, a transmembrane component of the flagellar type III protein export apparatus in Salmonella.
    Inoue, Y., Kinoshita, M., Namba, K. & Minamino, T.
    Genes Cells 
    in press 
    (2019)
    PMID: 30963674
  • Structural insights into the substrate specificity switching mechanism of the type III protein export apparatus.
    Inoue, Y., Ogawa, Y., Kinoshita, M., Shimada, M., Kodera, N., Ando, T., Namba, K, Kitao, A., Imada, K. & Minamino, T.
    Structure 
    in press 
    (2019)
  • Novel insights into conformational rearrangements of the bacterial flagellar switch complex.
    Sakai, T., Miyata, T., Terahara, N., Mori, K., Inoue, Y., Morimoto, Y.V., Kato, T., Namba, K. & Minamino, T.
    mBio 
    10, e00079-19, 2019 
    (2019)
    PMID: 30940700
  • Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment.
    Minamino, T., Terahara, N., Kojima, S. & Namba, K.
    Mol. Microbiol. 
    10, 723-734 
    (2018)
    PMID: 30069936
  • In vitro reconstitution of functional type III protein export and insights into flagellar assembly.
    Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T. & Imada, K.
    mBio 
    9(3), e00988-18 
    (2018)
    PMID: 29946050
  • The mechanism of two-phase motility in the spirochete Leptospira: Swimming and crawling.
    Tahara, H., Takabe, K., Sasaki, Y., Kasuga, K., Kawamoto, A., Koizumi, N., Nakamura, S.
    Sci. Adv. 
    4(5), eaar7975 
    (2018)
    PMID: 29854948
  • Leptospiral flagellar sheath protein FcpA interacts with FlaA2 and FlaB1 in Leptospira biflexa.
    Sasaki, Y., Kawamoto, A., Tahara, H., Kasuga, K., Sato, R., Ohnishi, M., Nakamura, S., Koizumi, N.
    PLoS One 
    13(4):e0194923 
    (2018)
    PMID: 29634754
  • Hierarchical protein export mechanism of the bacterial flagellar type III protein export apparatus.
    Minamino, T.
    FEMS Microbiol. Lett. 
    365, 1-9 
    (2018)
    PMID: 29850796
  • Technical Development of Electron Cryomicroscopy and Contributions to Life Sciences.
    Namba, K., Kato, T.
    JEOL NEWS 
    53, 18-24 
    (2018)
  • Electron Microscopy of Motor Structure and Possible Mechanisms.
    Minamino, T., Kato, T., Makino, F., Horvath, P., Miyata, T. & Namba, K.
    Encyclopedia of Biophysics 
    in press 
    (2018)
  • The bacterial flagellum.
    Minamino, T., Morimoto, Y.V., Kawamoto, A., Terashima, H. & Imada, K.
    Intech 
    in press 
    (2018)
  • Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export.
    Terahara, N., Inoue, Y., Kodera, N., Morimoto, Y.V., Uchihashi, T., Imada, K., Ando, T., Namba, K. & Minamino, T.
    Sci. Adv. 
    4(4), eaao7054 
    (2018)
    PMID: 29707633
  • Effect of a clockwise-locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor.
    Kinoshita, M., Namba, K. & Minamino, T.
    Genes Cells 
    23, 241-247 
    (2018)
    PMID: 29405551
  • Evidence for the hook supercoiling mechanism of the bacterial flagellum.
    Fujii, T., Matsunami, H., Inoue, Y. & Namba, K.
    Biophysics and Physicobiology 
    15, 28-32 
    (2018)
    PMID: 29607277
  • Novel insights into the mechanism of well-ordered assembly of bacterial flagellar proteins in Salmonella.
    Inoue, Y., Morimoto, Y.V., Namba, K. & Minamino, T.
    Sci Rep 
    1.574305556 
    (2018)
    PMID: 29379125
  • Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor.
    Kinoshita, M., Furukawa, Y., Uchiyama, S., Imada, K., Namba, K. & Minamino, T.
    BBRC  
    496, 12-17 
    (2018)
    PMID: 29294326
  • A triangular loop of domain D1 of FlgE is essential for hook assembly but not for the mechanical function.
    Sakai, T., Inoue, Y., Terahara, N., Namba, K. & Minamino, T.
    BBRC  
    495, 1789-1794 
    (2018)
    PMID: 29229393
  • Structural differences in the bacterial flagellar motor among bacterial species.
    Terashima, N., Kawamoto, A., Morimoto, Y.V., Imada, K. & Minamino, T.
    Biophysics and Physicobiology 
    14, 191-198 
    (2017)
    PMID: 29362704
  • Determination of local pH differences within living Salmonella cells by high-resolution pH imaging based on pH-sensitive GFP derivative, pHluorin(M153R).
    Morimoto, Y.V., Kami-ike, N., Namba, K. & Minamino, T.
    Bio-protocol 
    7(1): e2529 
    (2017)
  • Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.
    Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.
    Sci. Adv. 
    in press 
    (2017)
    PMID: 29109979
  • The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.
    Pourjaberi, S.N.S., Terahara, N., Namba, K. & Minamino, T.
    Mol. Microbiol. 
    in press 
    (2017)
    PMID: 28925530
  • Implications of coordinated cell-body rotations for Leptospira motility.
    Takabe, K., Kawamoto, A., Tahara, H., Kudo, S. & Nakamura, S.
    Biol. Open 
    491, 1040-1046 
    (2017)
    PMID: 28780349
  • Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex
    Fukumura T, Makino F, Dietsche T, Kinoshita M, Kato T, Wagner S, Namba K, Imada K, Minamino T
    PLoS. Biol. 
    15(8):e2002281 
    (2017)
    PMID: 28771466
  • Stoichiometry and turnover of the stator and rotor.
    Morimoto, Y.V., Minamino, T.
    The Bacterial Flagellum _ Methods and Protocols- 
    1593: 203-213 
    (2017)
    PMID: 28389956
  • Structural study of the bacterial flagellar basal body by electron cryomicroscopy and image analysis.
    Kawamoto, A., Namba, K.
    The Bacterial Flagellum _ Methods and Protocols- 
    1593: 119-131 
    (2017)
    PMID: 28389949
  • Fuel of the bacterial flagellar type III protein export apparatus.
    Kawamoto, A., Namba, K.
    The Bacterial Flagellum _ Methods and Protocols- 
    1593: 3-16 
    (2017)
    PMID: 28389941
  • Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE.
    Hiraoka, K.D., Morimoto, Y.V., Inoue, Y., Fujii, T., Miyata, T., Makino, F., Minamino, T. & Namba, K.
    Sci Rep 
    7:46723 
    (2017)
    PMID: 28429800
  • Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor.
    Terahara, N., Noguchi, Y., Nakamura, S., Kamiike, N., Ito, M., Namba, K., Minamino, T.
    Sci Rep 
    7:46081 
    (2017)
    PMID: 28378843
  • Bacterial flagella grow through an injection-diffusion mechanism.
    Renault, T.T., Abraham, A.O., Bergmiller, T., Parados, G., Rainville, S., Charpentier, E., Guet, C.C., Tu, Y., Namba, K., Keener, J.P., Minamino, T. & Erhardt, M.
    eLife 
    6:e23136 
    (2017)
    PMID: 28262091
  • Measurements of free-swimming speed of motile Salmonella cells in liquid media.
    Morimoto, Y.V., Namba, K. & Minamino, T.
    Bio-protocol 
    7(1): e2093 
    (2017)
  • Bacterial intracellular sodium ion measurement using CoroNa Green.
    Morimoto, Y.V., Namba, K. & Minamino, T.
    Bio-protocol 
    7(1): e2092 
    (2017)
  • Identical folds used for distinct mechanical functions of the bacterial flagellar rod and hook.
    Fujii T, Kato T, Hiraoka KD, Miyata T, Minamino T, Chevance FF, Hughes KT, Namba K
    Nat. Commun. 
    8:14276 
    (2017)
    PMID: 28120828
  • Structure of actomyosin rigour complex at 5.2-_ resolution and insights into the ATPase cycle mechanism
    Fujii T, Namba K.
    Nat. Commun. 
    8:13969 
    (2017)
    PMID: 28067235
  • High-resolution pH imaging of living bacterial cell to detect local pH differences.
    Morimoto YV, Kami-Ike N, Miyata T, Kawamoto A, Kato T, Namba K3,2, Minamino T
    mBio 
    7(6) 
    (2016)
    PMID: 27923921
  • The architecture of the cytoplasmic region of type III secretion systems.
    Makino, F., Shen, D., Kajimura, N., Kawamoto, A., Pissaridou, P., Oswin, H., Pain M., Murillo, I., Namba, K. & Blocker, A.J.
    Sci Rep 
    6:33341 
    (2016)
    PMID: 27686865
  • Structural stability of flagellin subunit affects the rate of flagellin export in the absence of FliS chaperone.
    Furukawa, Y., Inoue, Y., Sakaguchi, A., Mori, Y., Fukumura, T., Miyata, T., Namba, K. & Minamino, T.
    Mol. Microbiol. 
    in press 
     
    PMID: 27461872
  • The terameric MotA complex as the core of the flagellar motor stator from hyperthermophilic bacterium.
    Takekawa, N., Terahara, N., Kato, T., Gohara, M., Mayanagi, K., Hijikata, A., Onoue, Y., Kojima, S., Shirai, T., Namba, K. & Homma, M.
    Sci Rep 
    6:31526 
    (2016)
    PMID: 27531865
  • Structural study of MPN387, an essential protein for gliding motility of a human pathogenic bacterium, Mycoplasma pneumonia.
    Kawakita, Y., Kinoshita, M., Furukawa, Y., Tulum, I., Katayama, E., Namba, K. & Miyata, M.
    J. Bacteriol. 
    198, 2352-2359 
    (2016)
    PMID: 27325681
  • Structural flexibility of the periplasmic protein, FlgA, regulates flagellar P-ring assembly in Salmonella enterica.
    Matsunami, H., Yoon, Y.-H., Meshcheryakov, V.A., Namba, K. & Samatey, F.A.
    Sci Rep 
    6:27399 
    (2016)
    PMID: 27273476
  • Rearrangements of α-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.
    Kinoshita, M., Nakanishi, Y., Furukawa, Y., Namba, K., Imada, K. & Minamino, T.
    Mol. Microbiol. 
    101, 656-670 
    (2016)
    PMID: 27178222
  • Periodicity in attachment organelle revealed by electron cryotomography suggests conformational changes in gliding mechanism of Mycoplasma pneumoniae.
    Kawamoto, A., Matsuo, L., Kato, T., Yamamoto, H., Namba, K., Miyata, M.
    mBio 
    7(2): e00243-16 
    (2016)
    PMID: 27073090
  • Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.
    Imada, K., Minamino, T., Uchida, Y., Kinoshita, M., Namba, K.
    Proc. Natl. Acad. Sci. U. S. A. 
    113(13):3633-3638 
    (2016)
    PMID: 26984495
  • Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor.
    Baker, M. A. B., Hynson, R. M. G., Ganuelas, L. A., Mohammadi, N. S., Liew, C. W., Rey, A. A., Duff, A. P., Whitten, A. E., Jeffries, C. M., Delalez, N. J., Morimoto, Y. V., Stock, D., Armitage, J. P., Turberfield, A. J., Namba, K., Berry, R. M. & Lee, L. K.
    Nat. Struct. Mol. Biol. 
    23, 197-203 
    (2016)
    PMID: 26854663
  • The bacterial flagellar type III export gate complex is a dual fuel engine that can use both H+ and Na+ for flagellar protein export.
    Minamino, T., Morimoto, Y.V., Hara, N., Aldridge, P.D. , Namba, K.
    PLoS Pathog. 
    4;12(3):e1005495 
    (2016)
    PMID: 26943926
  • FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.
    Minamino, T., Kinoshita, M., Inoue, Y., Morimoto, Y.V., Ihara, K., Koya, S., Hara, N., Nishioka, N., Kojima, S., Homma, M. & Namba, K.
    MicrobiologyOpen 
    in press 
     
    PMID: 26916245
  • Genetic analysis of revertants isolated from the rod-fragile fliF mutant of Salmonella.
    Komatsu, H., Hayashi, F., Sasa, M., Shikata, K., Yamaguchi, S., Namba, K. & Oosawa, K.
    Biophysics and Physicobiology 
    13, 13-25 
    (2016)
    PMID: 27924254