Graduate School of Frontier Biosciences, Osaka University

Japanese

Colloquium 195

Speaker Masanari Seike (Assistant Professor, Laboratory of Stem Cell Biology and Developmental Immunology)
Title Stem cell niche-specific Ebf3 maintains the bone marrow cavity
Date Wed., October 10, 2018, 12:15~13:00
Place 2F Seminar room, BioSystems Building
Host Tatsuki Sugiyama (Associate Professor, Cellular and Molecular Neurobiology Group)
Tel: 06-6879-7968












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Abstract

Bone marrow is the tissue filling the space between bone surfaces. Hematopoietic stem cells (HSCs) are maintained by special microenvironments known as niches within bone marrow cavities. Mesenchymal cells, termed CXC chemokine ligand 12 (CXCL12)-abundant reticular (CAR) cells, are a major cellular component of HSC niches that gives rise to osteoblasts in bone marrow. However, it remains unclear how osteogenesis is prevented in most CAR cells to maintain HSC niches and marrow cavities. Here, using lineage tracing, we found that the transcription factor early B-cell factor 3 (Ebf3) is preferentially expressed in CAR cells and that Ebf3-expressing cells are self-renewing mesenchymal stem cells in adult marrow. When Ebf3 is deleted in CAR cells, HSC niche function is severely impaired, and bone marrow is osteosclerotic with increased bone in aged mice. In mice lacking Ebf1 and Ebf3, CAR cells exhibiting a normal morphology are abundantly present, but their niche function is markedly impaired with depleted HSCs in infant marrow. Subsequently, the mutants become progressively more osteosclerotic, leading to the complete occlusion of marrow cavities in early adulthood. CAR cells differentiate into bone-producing cells with reduced HSC niche factor expression in the absence of Ebf1/Ebf3. Thus, HSC cellular niches express Ebf3 that is required to create HSC niches, to inhibit their osteoblast differentiation, and to maintain spaces for HSCs.

Reference

  1. Seike, M. et al., Genes Dev. 32, 359 (2018).
  2. Shimoto, M. et al., Blood 129, 2124 (2017).
  3. Omatsu, Y. et al., Nature 508, 536 (2014).
  4. Omatsu, Y. et al., Immunity 33, 387 (2010).
  5. Sugiyama, T. et al., Immunity 25, 977 (2006).

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