Graduate School of Frontier Biosciences, Osaka University

Japanese

Colloquium 186

The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM

Speaker Takayuki Kato (specially appointed associate professor, Namba Lab.)
Title The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM
Speaker Tomoko Miyata (specially appointed assistant professor, Namba Lab.)
Title The structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM
Date Wed., May 30, 2018, 12:15~13:00
Place 2F Seminar room, BioSystems Building
Host Takayuki Kato (specially appointed associate professor, Namba Lab.)
Tel: 06-6879-4625
E-mail: galy[at]fbs.osaka-u.ac.jp

Abstract

X-ray crystallography, NMR, and electron cryomicroscopy (cryoEM) are important techniques for structural analysis of biological macromolecules. Each technique has its own advantages and disadvantages but cryoEM is the most versatile in terms of sample state and condition and can be used in a far wider range of specimens than the other two. CryoEM was not so popular than others until a few years ago because generally attainable resolution was limited to around 10 Å. However, the resolution was dramatically improved by the recent development of direct electron detection camera. That is why macromolecular structures solved by cryoEM image analysis are found in top level journals, such as Nature and Science, in almost every issue. The Nobel Prize in Chemistry was awarded to three pioneers in the cryoEM field because of the present power and usefulness of the technique for life and medical sciences. In this colloquium, we will describe the structural analysis of the bacterial flagella motor, the development of a new cryoTEM, and a potential future of cryoEM.


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