Graduate School of Frontier Biosciences, Osaka University

Japanese

Colloquium 240

Speaker Yoshioka Daisuke (Specially Appointed Researcher of Integrative Physiology (FBS student ~2020.3.31) )
Title PTEN-PI(4,5)P2 positive feedback for stabilizing asymmetric PI(3,4,5)P3 pattern in migrating cell.
Date Thu., June. 11, 2020, 12:15~13:00
Place Zoom (on line):We will announce the Meeting URL, ID and Password until the day before the each colloquium by Email.
Host Name:Shuji Tachibanaki
Tel :06-6879-4611
E-mail:banaki@fbs.osaka-u.ac.jp
Language Japanese

Title/Abstract

PTEN-PI(4,5)P2 positive feedback for stabilizing asymmetric PI(3,4,5)P3 pattern in migrating cell.

In order for a cell to move, it is necessary to form asymmetric signal in the cell. PI(3,4,5)P3, a phosphatidylinositol lipid, is well known as one of the major signaling molecules responsible for cell polarization. Since PI(3,4,5)P3 promotes the formation of pseudopods, the region where PI(3,4,5)P3 is localized become the “anterior” of the cells. Conversely, PTEN, a 3-phosphatase of PI(3,4,5)P3, acts on the “posterior” region for suppression of PI(3,4,5)P3 production. However, the mechanism for the membrane accumulation of PTEN hasremained to be clarified. Here, we developed an in vitro single-molecule imaging assay system to analyze the interaction between PTEN and lipids. Single-molecule imaging revealed that PTEN’s membrane binding is stabilized by its enzymatic product, PI(4,5)P2. PTEN-PI(4,5)P2 interaction enabled efficient cell migration through the PI(3,4,5)P3 distribution restricted to the anterior. These results demonstrate that a PI(4,5)P2-mediated positive feedback mechanism for the PTEN accumulation underlies the anterior-posterior polarization in motile cells.


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