Graduate School of Frontier Biosciences, Osaka University

Japanese

Colloquium 217

Speaker Yohei Kirino (Associate Prof., Computational Medicine Center, Department of Biochemistry and Molecular Biology, Thomas Jefferson University)
Title Expression and function of cyclic phosphate-containing RNAs: a hidden layer of the transcriptome
Date Tue., July 16, 2019, 15:00〜16:00
Place 2F Seminar room, BioSystems Building
Host Name: Toshie Kai (Full Professor, FBS Germline Biology Group)       
Tel : 06-6879-4645 (FBS planning office)
E-mail: fbs-kikaku@fbs.osaka-u.ac.jp
Language English












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Title/Abstract

Expression and function of cyclic phosphate-containing RNAs: a hidden layer of the transcriptome

Cellular RNA molecules contain phosphate or hydroxyl ends. A 2′,3′-cyclic phosphate (cP) is one of the 3′-terminal forms of RNAs mainly generated from RNA cleavage by ribonucleases. Although transcriptome profiling using RNA-seq has become a ubiquitous tool in biomedical research, cP-containing RNAs (cP-RNAs) form a hidden transcriptome layer, which is infrequently recognized and characterized, because standard RNA-seq is unable to capture them. Despite cP-RNAs’ invisibility in RNA-seq data, increasing evidence indicates that they are not accumulated simply as non-functional degradation products; rather, they have physiological roles in various biological processes, designating them as noteworthy functional molecules. By developing cP-RNA-seq, we revealed comprehensive cP-RNA expression repertoire in cell lines and tissues. In addition to the cP-RNA expression profiles, the role of cP-RNAs in asthma and infectious diseases will be presented and discussed.


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