Graduate School of Frontier Biosciences, Osaka University

Japanese

Colloquium 211

Speaker Tohru Minamino (Associate professor/FBS Protonic NanoMachine Research Group)
Title Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment
Date Thu., May. 16, 2019, 12:15~13:00
Place 2F Seminar room, BioSystems Building
Host Name:Tohru Minamino        
Tel :4625
E-mail:tohru@fbs.osaka-u.ac.jp
Language Japanese










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Title/Abstract

Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment

The bacterial flagellar motor is composed of a rotor and a transmembrane ion channel complex that acts as a stator unit. The ion channel complex consists of at least three structural parts: a cytoplasmic domain responsible for the interaction with the rotor, a transmembrane ion channel that forms a pathway for the transit of ions across the cytoplasmic membrane, and a peptidoglycan-binding (PGB) domain that anchors the stator unit to the peptidoglycan (PG) layer. A flexible linker connecting the ion channel and the PGB domain not only coordinates stator assembly with its ion channel activity but also controls the assembly of stator units to the motor in response to changes in the environment. When the ion channel complex encounters the rotor, the N-terminal portion of the PGB domain adopts a partially stretched conformation, allowing the PGB domain to reach and bind to the PG layer. The binding affinity of the PGB domain for the PG layer is affected by the force applied to its anchoring point and to the type of ionic energy source. In this colloquium, I will present current understanding of autonomous control mechanism of stator assembly in the bacterial flagellar motor.


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