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Collaborative institutes
Laboratory of Immune Regulation Chugai Pharmaceutical CO.,LTD.
Optical Nano Device Group
OMRON Corporation

Lab. Members

Externally Funded Research Scientis MATSUSHITA, Tomohiko TEL +81-6-6879-7970
Externally Funded Research Scientist NISHIKAWA, Takeo TEL +81-6-6879-7970
Secretary HANEDA, Yoshiko TEL +81-6-6879-7970

1 The key device for proteome analysis :
Protein chip High sensitivity and high accuracy are the keys to success

Recent advances in genome sciences and protein ultrasensitive detection technology have accelerated the progress of "proteome analysis" in which the whole proteins in cells and tissues are isolated and identified.
The identification of the proteins itself is not, however,enough to fundamentally understand living body systems, and in addition,the analysis of the whole biomolecular interactions is indispensable. Protein chip is extensively expected
now as the key device for analyzing those numerous biomolecular interactions in a high-throughput manner.The only type of protein chip commercially available at present is antibody chip on which immunoglobulin is immobilized as probe protein.
The commercialized antibody chip has a problem in its sensitivity and accuracy to
detect the specific binding of antigen. Fluorescence measurement is also widely used for the detection of protein interactions, but it has the problems of the inactivation of target protein resulting from the conjugation of fluorophore to the protein. Furthermore, it is pointed out that the detection system for the fluorometry must be large-sized and expensive inevitably.

2 Advanced technology :
Breakthrough by the protein imprint method and waveguide- coupled surface plasmon sensor

The goal of our research group is the development of the next-generation protein chip to resolve the above problems. In order to immobilize the probe proteins in a well-organized manner and high density without inactivation, we are developing the protein imprinting method such that the protein mold is produced on a substrate,
adapting our original technology of nano-size fabrication. Thus,optimizing the orientation and density of the probe protein,the sensitivity and accuracy are farther improved. For detection, we are utilizing SPR (Surface Plasmon Resonance)
method that has attracted attention in recent years for its high sensitivity.
Integrating SPR with optical waveguide technology, which is extensively used in
optical communications, we are developing the waveguide-coupled SPR sensor that will be accurate, sensitive,small-sized, and low-priced detection system without using bulk-type optics such as lenses and filters.

3 Challenge to the immobilization of the whole proteins from human full- length cDNA : Application to the clinical medicine by the human protein chip

With the protein chip technology mentioned above,a highly sensitive protein chip is to be developed and then, applied to the clinical medicine.Domains forming regular rigid structures are fused to C or N terminal of proteins generated from the human full-length cDNA in a non-cell protein expression system by protein and gene
engineering. The molds of such domains are fabricated on the substrate to immobilize them. It is expected that this human protein chip will be widely used for the identification of disease factor proteins, elucidation of the disease mechanism,
the drug discovery, and so forth. By integrating technologies in the different fields such as nano fabrication, light wave control, proteomics and bioengineering,
we are aiming at the realization of the next-generation protein chip which has high sensitivity and accuracy. It is expected that our protein chip will spread widely in medical places, drug design development, home life and so on, and contribute to the medical treatment tailored to individual patients, establishment of the genome-
based drug discovery, and the spread of the preventive medical treatments.

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