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Biophysical Dynamics Laboratories Functional Proteomics Group (Prof. Norioka)
This laboratory has been closed as of August 2006. These pages are kept for historical records.
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Laboratory of Immune Regulation Chugai Pharmaceutical CO.,LTD.
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OMRON Corporation

  Lab. Members

Naoko NORIOKA

Research Fellow
Hiroshi SEZAKI

D3
Carlos Castillo Corbella

D2
Dissanayake Kumara Kithsiri

D1

  Publications

  1. Takeshi Ishimizu, Shigemi Norioka, Tetsu Nakanishi, and Fumio Sakiyama. (1998) S-genotype of 'Hosui' of Japanese pear is S3S5. J. Japan. Soc. Hort. Sci. 67(1), 35-38.

  2. Takeshi Ishimizu, Toyohide Shinkawa, Fumio Sakiyama, and Shigemi Norioka. (1998) Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility. Plant Mol. Biol. 37, 931-941.

  3. Hiroshi Yoshida, Mari Iizuka, Naoko Norioka, Shigemi Norioka, and Fumio Sakiyama. (1998) Cloning and sequencing of cDNA encoding ribonuclease F1 from Fusarium moniliforme. Biochemistry and Molecular Biology International 45, 555-560.

  4. Shaoliang Li, Shigemi Norioka, and Fumio Sakiyama. (1998) Bacteriolytic activity and specificity of Achromobacter beta-lytic protease. J. Biochem. 124, 332-339.

  5. Takeshi Ishimizu, Toshinori Endo, Yumi Yamaguchi-Kabata, Kazuo T. Nakamura, Fumio Sakiyama, and Shigemi Norioka. (1998) Identification of regions in which positive selection may operate in S-RNase of Maloideae in Rosaceae: Implication for S-allele-specific recognition sites in S-RNase. FEBS Letters 440, 337-342.

  6. Shigemi Norioka, Takeshi Ishimizu, and Fumio Sakiyama. (1998) Japanese Pear Self-incompatibility and ribonuclease - Self and nonself recognition between pistil and pollen. Report for Research Center of Protein Engineering 19, 22-28.

  7. Shigemi Norioka and Fumio Sakiyama. (1998) "Trypsin (Streptomyces erythraeus)" in Handbook of Proteolytic Enzymes (eds. Alan J. Barrett, Neil D. Rawlings and J. Fred Woessner), Academic Press London, pp. 22-24.

  8. Shigemi Norioka. (1998) "Primary Structure Analysis by Protein Sequencer" in Protocal of Experiments for Plant Proteins (eds. by Kenzo Nakamura, Mikio Nishimura, Toshiharu Hase and Masayoshi Maeshima), Shujunsha, pp. 179-188.

  9. Shigemi Norioka. (1998) "Digestion of Protein" in Story of Proteins which Support Our Lives (ed. by Protein Research Foundation), Kagakudojin, pp.26-35.

  10. Daiji Kiyozumi, Takeshi Ishimizu, Tetsu Nakanishi, Fumio Sakiyama, and Shigemi Norioka. (1999) Molecular cloning and nucleotide sequencing of a cDNA encoding UDP-glucose pyrophosphorylase of Japanese pear (Pyrus pyrifolia Nakai) (Accession No. AB013353). Plant Physiology 119, 363.

  11. Akiko Sasaki, Mai Yamagishi, Tomohiro Mega, Shigemi Norioka, Shunji Natsuka, and Sumihiro Hase. (1999) Partial purification and characterization of a novel endo-β-mannosidase acting on N-linked sugar chains from Lilium longflorum Thumb. J. Biochem. 125, 363-367.

  12. Shigemi Norioka. (1999) Recognition of Self and Non-self in Fertilization of Higher Plants. The Production and Technology 51(3), 44-47.

  13. Shigemi Norioka. (1999) "Microsequencing of Proteins" in Notes on Protein Experiments, Vol.2, from Separation and Identification to Protein Sequencing

    (eds. Kaoru Miyazaki and Masato Okada), Yodosha, pp. 71-82.
  14. Shaoliang Li, Shigemi Norioka, and Fumio Sakiyama. (2000) Purification, characterization and primary structure of a novel cell wall hydrolytic amidase, CwhA, from Achromobacter lyticus. J. Biochem. 127, 1033-1039.

  15. Niranjali Gamage, Takanori Matsuura, Naoko Norioka, Yumi Yoshimura, Takeshi Takasaki, Tetsu Nakanishi, and Shigemi Norioka. (2000) Expression of a fusion protein of Pyrus pyrifolia S-RNase with glutathione-S-transferase in E. coli. Biotechnology Letters 22, 1413-1417.

  16. Takanori Matsuura, Hiroaki Sakai, Masaki Unno, Koh Ida, Mamoru Sato, Fumio Sakiyama, and Shigemi Norioka. (2001) Crystal structure at 1.5-ナ resolution of Pyrus pyrifolia pistil ribonuclease responsible for gametophytic self-incompatibility. J. Biol. Chem. 276, 45261-45269.

  17. Koh Ida, Shigemi Norioka, Masaki Yamamoto, Takashi Kumasaka, Eiki Yamashita, Ed Newbigin, Adrienne E. Clarke, Fumio Sakiyama, and Mamoru Sato. (2001) The 1.55 ナ resolution structure of Nicotiana alata SF11-RNase associated with gametophytic self-incompatibility. J. Mol. Biol 314, 103-112.

  18. Naoko Norioka, Hironori Katayama, Tohru Matsuki, Takeshi Ishimizu, Tetsu Nakanishi, and Shigemi Norioka. (2001) Sequence comparison of the 5' flanking regions of Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility. Sex. Plant Reprod. 13, 289-291.

  19. Carlos Castillo, Takeshi Takasaki, Toshihiro Saito, Yumi Yosimura, Shigemi Norioka, and Tetsu Nakanishi. (2001) Reconsideration of S-genotype assignments, and discovery of a new allele based on S-RNase PCR-RFLPs in Japanese pear cultivars. Breeding Science 51, 5-11.

  20. Koh Ida, Toyohide Shinkawa, Shigemi Norioka, Ed Newbigin, Adrienne E. Clarke, Fumio Sakiyama, and Mamoru Sato. (2001) Crystallization and preliminary X-ray crystallographic analysis of S-allelic glycoprotein SF11-RNase from Nicotiana alata. Acta Crystallographica D 57, 143-144.

  21. Takanori Matsuura, Masaki Unno, Hiroaki Sakai, Tomitake Tsukihara, and Shigemi Norioka. (2001) Purification and crystallization of Japanese pear S-RNase associated with gametophytic self-Incompatibility. Acta Crystallographica D 57, 172-173.

  22. Shigemi Norioka. (2001) "Automatic Protein Sequence Analysis" in Methods for Basic Biochemical Experiments (eds. by Tairo Ohshima, Yuzuru Ishimura, Takao Shimizu, Naoyuki Taniguchi, Takeshi Mizuno and Kimitsuna Watanabe), Tokyo Kagaku Dojin, pp.143-152.

  23. Shigemi Norioka, Tetsu Nakanishi, and Fumio Sakiyama. (2001) "Self-incompatibility in Japanese Pear" in Flowers: Molecular Biology of Sex and Reproduction (eds. by Kokichi Hinata, Akira Isogai, Hiroshi Kamata, Yoshihumi Yomeda, Yashuo Nagato and Kinya Toriyama), Gakkai Shuppan Center, pp. 213-226.

  24. Daiji Kiyozumi, Takeshi Ishimizu, Shaoliang Li, Tetsu Nakanishi, and Shigemi Norioka. (2002) Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to S genotype of Pyrus pyrifolia, Japanese pear. Sex. Plant Reprod, in press.

  25. Katsuhiko Kondo, Mayu Yamamoto, Daniel P. Matton, Takanori Sato, Shigemi Norioka, Tsukaho Hattori, and Yasuo Kowyama. (2002) Cultivated tomato has defects in both S-RNase and HT protein genes required for stylar function of self-incompatibility. Plant Journal, in press.

  26. Yuegang Zhou, Naoko Norioka, Shaoliang Li, Shigemi Norioka. (2002) Nucleotide sequence of a cDNA encoding plant defensin-like protein (PDN1) in pollen grains of Japanese pear (Pyrus pyrifolia). Journal of Plant Physiology and Molecular Biology in China, in press.

  27. Carlos Castillo, Takeshi Takasaki, T. Saito, T. Ishimizu, Shigemi Norioka, and Tetsu Nakanishi. (2002) S-RNase based PCR-RFLP system for S-genotype identification in Japanese pear. Acta Horticulture, in press.

  28. D.M.R.K.K. Dissanayake, Takeshi Takasaki, Naoko Norioka, Shigemi Norioka, and Tetsu Nakanishi. (2002) Cis-regulatory elements for pistil specific expression in S-RNase promoter region of Japanese pear. Acta Horticulture, in press.

  29. Kentaro Shiraki, Shigemi Norioka, Shaoliang Li, and Fumio Sakiyama. (2002) Contribution of an Imidazole-Indole Stack to High Catalytic Potency of a Lysine-Specific Serine Protease, Achromobactor Protease I. J. Biochem 131(2), 213-218.

  30. Carlos Castillo, Takeshi Takasaki, Toshihiro Saito, Shigemi Norioka, and Tetsu Nakanishi. (2002) Cloning of the S8-RNase (S8-allele) of Japanese pear (Pyrus pyrifolia Nakai). Plant Biotechnology 19(1), 1-6.

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