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Name |
Email |
Telephone |
| Associate Prof |
MASUTANI,Chikahide,D.Pharm. |
 |
+81-6-6879-7978 |
| FAX |
+81-6-6877-9382 |
| Postal Mail Address |
Graduate School of Frontier Biosciences, Osaka
University,
1-3 Yamada-oka, Suita, Osaka, 565-0871, Japan |
| For more information |
|
Major projects engaged in by this Division
are:.
| 1 |
Molecular mechanism
of nucleotide excision repair. |
| In the course of evolution, living organisms
acquired various kinds of DNA repair mechanism. Nucleotide
excision repair is one of the most versatile DNA repair
systems, which eliminates a wide variety of DNA damage.
We constructed a cell-free DNA repair system with UV-irradiated
SV40 minichromosomes as templates. Using this system,
we are trying toE^purify proteins involved in nucleotide
excision repair in mammalian cells and to clarify the
mode of actions of these protein factors. |
| 2 |
Studies on general transcription
factors. |
| Eukaryotic transcription is supported by
three distinct RNA polymerases and, especially, RNA
polymerase II (Pol II) involves in gene expression.
Through the analyses of the general transcription factor
TFIIH, which involves coodinative regulation between
Pol II transcription and nucleotide excision repair,
and another general transcription factor TFIIE that
regulates TFIIH, we have been studying molecular mechanisms
of transcription and repair. |
| 3 |
Mechanisms of chromosome
replication. |
| DNA replication is the most fundamental
process for cell duplication and locates in the center
of cell cycle. We have been analyzing the structure
and function of mammalian DNA polymerase D.Transcriptional
regulation during the cell cycle of genes for DNA polymerase
D subunits is also investigated. In addition, we are
working on a novel DNA polymerase which can bypass
DNA damages. |
| |
Three Step Model for
Transcription Initiation |
| At the first step, before transcription
initiation, the promoter DNA is opened in the preinitiation
complex. At the second step, DNA opening expands to
downstream during transcription initiation. At the
third step, Pol II forms an elongation complex and
moves downstream together with opened DNA position.
The abbreviations: B,TFIIB; D,TFIID; E,TFIIE; F,TFIIF;
H,TFIIH; Pol IIA, hypophosphorylated Pol II; Pol IIO,
phosphorylated Pol II. |
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Damage Recognition and
Initiation of Nucleotide Excision Repair by XPC Protein
Complex |
| In combination with DNase I footprinting,
DNA damage recognition-competition assay, and coimmunoprecipitation
assay, we clearly demonstrated that XPC-hHR23B plays
a central role in damage detection and initiation of
the NER reaction. |
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