Graduate School of Frontier Biosciences, Osaka University


Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains

Journal J Cell Biol 203, 417-425 (2013)
Authors Kazuhiro Tateishi (1), Yuji Yamazaki (1), Tomoki Nishida (2), Shin Watanabe (1), Koshi Kunimoto (3), Hiroaki Ishikawa (4), Sachiko Tsukita (1)*

  1. Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan
  2. Research Center for Ultra-high Voltage Electron Microscopy, Osaka University, Osaka 567-0047, Japan
  3. Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305
  4. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158

K. Tateishi and Y. Yamazaki contributed equally to this paper.
*: Corresponding author
Title Two appendages homologous between basal bodies and centrioles are formed using distinct Odf2 domains
PubMed 24189274
Laboratory Biological Science Group 〈Prof. Tsukita〉
Description Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and basal feet. However, the molecular basis for this regulation is not fully understood. The Odf2 gene product, ODF2/cenexin, is essential for both ciliogenesis and the formation of the distal and subdistal appendages on centrioles, which become basal bodies. We examined the effects of Odf2 deletion constructs on ciliogenesis in Odf2-knockout F9 cells. Electron microscopy revealed that ciliogenesis and transition fiber formation required the ODF2/cenexin fragment containing amino acids (aa) 188-806, whereas basal foot formation required aa 1-59 and 188-806. These sequences also formed distal and subdistal appendages, respectively, indicating that the centriole appendages are molecularly analogous to those on basal bodies. We used the differential formation of appendages by Odf2 deletion constructs to study the incorporation and function of molecules associated with each appendage type. We found that transition fibers and distal appendages were required for ciliogenesis and subdistal appendages stabilized the centrosomal microtubules.