Graduate School of Frontier Biosciences, Osaka University

Japanese

An in vitro reproduction of stress-induced memory defects: Effects of corticoids on dendritic spine dynamics

Journal Sci Rep 6, 19287 (2016)
Authors Saito S (1), Kimura S (1), Adachi N (2), Numakawa T (2), Ogura A (1), Tominaga-Yoshino K (1)

  1. Laboratory of Synaptic Plasticity, Osaka University Graduate School of Frontier Biosciences, Suita, Osaka 565-0871 Japan.
  2. National Center of Neurology and Psychiatry, Kodaira 187-8551 Tokyo, Japan.
Title An in vitro reproduction of stress-induced memory defects: Effects of corticoids on dendritic spine dynamics
PubMed 26765339
Laboratory Synaptic Plasticity Group 〈Assoc. Prof. Tominaga-Yoshino〉
Abstract Previously, in organotypic slice culture of rodent hippocampus we found that three repeated inductions of LTP, but not a single induction, led to a slow-developing long-lasting enhancement of synaptic strength coupled with synapse formation. Naming this structural plasticity RISE (repetitive LTP-induced synaptic enhancement) and assuming it to be a potential in vitro reproduction of repetition-dependent memory consolidation, we are analyzing its cellular mechanisms. Here, we applied a glucocorticoid to the culture to mimic acute excess stress and demonstrated its blockade of RISE. Since excess stress interferes with behavioral memory consolidation, the parallelism between RISE in vitro and memory consolidation in vivo is supported. We recently reported that RISE developed after stochastic processes. Here we found that the glucocorticoid interfered with RISE by suppressing the increment of dendritic spine fluctuation that precedes a net increase in spine density. The present study provides clues for understanding the mechanism of stress-induced memory defects.