Graduate School of Frontier Biosciences, Osaka University

Japanese

Epigenetically induced paucity of histone H2A.Z stabilizes fission yeast ectopic centromeres

Journal Nat Struct Mol Biol 20, 1397-1406 (2013)

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Authors Yuki Ogiyama, Yuko Ohno, Yoshino Kubota, Kojiro Ishii
(Graduate School of Frontier Biosciences, Osaka University 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan)
Title Epigenetically induced paucity of histone H2A.Z stabilizes fission yeast ectopic centromeres
PubMed 24186062
Laboratory
Abstract In most eukaryotes, centromeres are epigenetically defined by nucleosomes that contain the histone H3 variant centromere protein A (CENP-A). Specific targeting of the CENP-A-loading chaperone to the centromere is vital for stable centromere propagation; however, the existence of ectopic centromeres (neocentromeres) indicates that this chaperone can function in different chromatin environments. The mechanism responsible for accommodating the CENP-A chaperone at non-centromeric regions is poorly understood. Here, we report the identification of transient, immature neocentromeres in Schizosaccharomyces pombe that show reduced association with the CENP-A chaperone Scm3, which is attributable to persistence of the histone H2A variant H2A.Z. Following the acquisition of adjacent heterochromatin or relocation of the immature neocentromeres to subtelomeric regions, H2A.Z was depleted and Scm3 was replenished, leading to subsequent stabilization of the neocentromeres. These findings provide novel insights into histone variant-mediated epigenetic control of neocentromere establishment.