Graduate School of Frontier Biosciences, Osaka University

Japanese

Chromosome-associated RNA-protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe

Journal Nat Commun 10, 5598 (2019)
Authors Ding DQ (1), Okamasa K (1), Katou Y (2), Oya E (3, 4), Nakayama JI (3, 5), Chikashige Y (1), Shirahige K (2), Haraguchi T (1, 6), Hiraoka Y (1, 6)
  1. Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, 651-2492, Japan.
  2. Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, 113-0032, Japan.
  3. Graduate School of Natural Sciences, Nagoya City University, Nagoya, 467-8501, Japan.
  4. Faculty of Science and Engineering, Chuo University, Tokyo, 112-8551, Japan.
  5. Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, 444-8585, Japan.
  6. Graduate School of Frontier Biosciences, Osaka University, Suita, 565-0871, Japan.
Title Chromosome-associated RNA-protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe
PubMed 31811152
Laboratory Nuclear Dynamics Group 〈Prof. Hiraoka〉
Abstract Pairing of homologous chromosomes in meiosis is essential for sexual reproduction. We have previously demonstrated that the fission yeast sme2 RNA, a meiosis-specific long noncoding RNA (lncRNA), accumulates at the sme2 chromosomal loci and mediates their robust pairing in meiosis. However, the mechanisms underlying lncRNA-mediated homologous pairing have remained elusive. In this study, we identify conserved RNA-binding proteins that are required for robust pairing of homologous chromosomes. These proteins accumulate mainly at the sme2 and two other chromosomal loci together with meiosis-specific lncRNAs transcribed from these loci. Remarkably, the chromosomal accumulation of these lncRNA-protein complexes is required for robust pairing. Moreover, the lncRNA-protein complexes exhibit phase separation properties, since 1,6-hexanediol treatment reversibly disassembled these complexes and disrupted the pairing of associated loci. We propose that lncRNA-protein complexes assembled at specific chromosomal loci mediate recognition and subsequent pairing of homologous chromosomes.