Graduate School of Frontier Biosciences, Osaka University

Japanese

Fluctuation Analysis of Mechanochemical Coupling Depending on the Type of Biomolecular Motors

Journal Physical Review Letters 101, 128103 (2008)
Authors Masatoshi Nishikawa, Hiroaki Takagi, Tatsuo Shibata, AtsukoH. Iwane, and Toshio Yanagida
Title Fluctuation Analysis of Mechanochemical Coupling Depending on the Type of Biomolecular Motors
PubMed 18851416
Laboratory
Abstract Mechanochemical coupling was studied for myosin II and V consistently. The fluctuation in myosin V motility was determined by correlating the stochasticity of the ATPase reaction with regular displacements per one ATP, consistent with a tight mechanochemical coupling. In contrast, myosin II, working in an ensemble, was explained by a loose coupling, generating variable step sizes which depend on [ATP] and realizing a much larger step (200 nm) per one ATP than myosin V through its cooperativity at zero load. These different mechanics are ideal for their physiological functions.