Graduate School of Frontier Biosciences, Osaka University

Japanese

Coordinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet

Journal Cell 148, 189-200 (2012)
Authors Koshi Kunimoto (1), Yuji Yamazaki (1,*), Tomoki Nishida (2,*), Kyosuke Shinohara (3,*), Hiroaki Ishikawa (4,*), Toshiaki Hasegawa (2), Takeshi Okanoue (5), Hiroshi Hamada (3), Tetsuo Noda (6), Atsushi Tamura (1), Shoichiro Tsukita (7) and Sachiko Tsukita (1,§)
  1. Laboratory of Biological Science, Graduate School of Frontier Biosciences and Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
  2. Research Center for Ultra-high Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
  3. Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University and CREST, Japan Science and Technology Corporation (JST), 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.
  4. Department of Biochemistry and Biophysics, University of California, San Francisco, CA94143, USA
  5. Department of Hepatology, Saiseikai Suita Hospital, Osaka, Japan.
  6. Department of Cell Biology, Cancer Institute of Japanese Foundation for Cancer Research, 3-10-6 Ariake, Koto-ku, Tokyo 135-8550,Japan.
  7. Department of Cell Biology, Faculty of Medicine, Kyoto University, Yoshida-Konoe, Kyoto 606-8315, Japan.

* : These authors nearly equally contribute to this work from different directions.
§ : Corresponding author.
Title Coordinated Ciliary Beating Requires Odf2-Mediated Polarization of Basal Bodies via Basal Feet
PubMed 22265411
Laboratory Biological Science Group 〈Prof. Tsukita〉
Abstract Coordinated beating of cilia in the trachea generates a directional flow of mucus required to clear the airways. Each cilium originates from a barrel-shaped basal body, from the side of which protrudes a structure known as the basal foot. We generated mice in which exons 6 and 7 of Odf2, encoding a basal body and centrosome-associated protein Odf2/cenexin, are disrupted. Although Odf2(ΔEx6,7/ΔEx6,7) mice form cilia, ciliary beating is uncoordinated, and the mice display a coughing/sneezing phenotype. Whereas residual expression of the C-terminal region of Odf2 in these mice is sufficient for ciliogenesis, the resulting basal bodies lack basal feet. Loss of basal feet in ciliated epithelia disrupted the polarized organization of apical microtubule lattice without affecting planar cell polarity. The requirement for Odf2 in basal foot formation, therefore, reveals a crucial role of this structure in the polarized alignment of basal bodies and coordinated ciliary beating.