Graduate School of Frontier Biosciences, Osaka University

Japanese

Brownian search-and-catch mechanism for myosin-VI steps

Journal Nat Chem Biol 5, 403-405 (2009)
Authors M. Iwaki, A.H. Iwane, T. Shimokawa, R. Cooke, T. Yanagida
Title Brownian search-and-catch mechanism for myosin-VI steps
PubMed 19430485
Laboratory
Abstract The cargo transporter myosin-VI processively walks along actin filaments using its two heads. Here we use single-molecule nanometry to show that the strong binding by myosin heads to actin is greatly accelerated (approximately 30-fold) when backward strain is applied to weakly bound heads during the actin search. We propose that the myosin head searches for the forward actin target by Brownian motion and catches the actin in a strain-dependent manner.