Report on ASM Conference on DNA Repair & Mutagenesis:
From Molecular Structure to Biological Consequences
Yasunori Fukumoto (Hanaoka laboratory)
Destination: The Fairmont Southampton Princess, Bermuda
Purpose: to participate in ASM Conference on DNA Repair and Mutagenesis,
make poster presentation and learn the latest knowledge and recent trend
of this field to help myself determine future career.
11/13 Itami ¨ Narita ¨ JFK (U.S.A.)
11/14 JFK (U.S.A.) ¨ Bermuda (U.K.)
11/14-19 ASM Conference on DNA Repair & Mutagenesis, the Fairmont
11/20-21 Bermuda (U.K.) ¨ JFK (U.S.A.) ¨ Narita ¨ Itami
Meeting report and Perspective
As one of the biggest meetings of this field, this conference is held
approximately every four years and it was a shame that this conference
was postponed last year because of a hurricane. Accompanied with around
six hundred participants and more than five hundred abstracts for poster
session, the conference began with keynote address by Phil Hanawalt
followed by gmusich session by famous professors in at-home mood, which
last rest of the meeting. In the oral session, I noticed that many
researchers work on Bacteria and yeasts, which seem to be rather less
respected in domestic meetings. Every one of those talks was impressive,
and itfs good to know that because I myself work on yeast.
Currently I work on DDB1 homologue (ddb1+) in fission yeast. DDB1 was
originally isolated as nucleotide excision factor, but recent research
suggests that DDB1 composes ubiquitin ligase (E3) and involves in various
cellular processes through E3 activity. DDB1 requires additional factors
to ubiquitinate target proteins, therefore it is important to identify
DDB1-interacting proteins in vivo. Fortunately, I had started to purify
Ddb1 complex from fission yeast ages ago, and in this conference, I presented
the result of purification for poster session together with genetical
analyses of ddb1+. I have at least two competing groups in U.S.A. and
I had expected them to visit my poster, but both of them were absent
in this conference, which relieved but also disappointed me.
Attending this meeting, I hope to find some clue for my future career.
For that purpose, I felt that I need to know what the less understood
things are, and I think I found some. For example, UV tolerance system
other than translesion synthesis and replication recovery from DNA damage,
which are currently recognized and analyzed in E. coli, and proposed
but not well defined for eukaryotes. And in this conference, I felt we
had a few sessions and posters about repair of interstrand crosslinks,
which is one of the most highly toxic DNA lesions and concerned with
some of the antineoplastic regent, but the mechanism of its removal is
poorly understood compared with other excision repair machineries and
so on, maybe because of the difficulty in analyses in vivo.
By the way, Bermuda is suitable place for conference with warm climate
and tropical beaches. But it seemed more than suitable, because around
the middle of the conference period, we had many skippers who might have
been on the nearby beach, enjoying the sunshine.